PXD017299-1

PXD017299 is an original dataset announced via ProteomeXchange.

Title	Influence of cross-linker polarity on selectivity towards lysine side chains
Description	Even though the amine reactive BS2G and DSG cross-linkers have the same length of spacer and are based on N-hydroxysuccinimidic group, our data showed that each of them formed preferentially different cross-links. We demonstrated that the choice of cross-linker can have a significant impact on the output data for structural characterization of biomolecules. Using equimolar mixtures of DSG with d6-BS2G, and BS2G with d6-DSG, we established that the polar BS2G preferentially bound to polar regions of modified molecule, whereas non-polar DSG bound to hydrophobic regions. This phenomenon established that the mixture of polar and non-polar cross-linkers acted as an efficient tool for the determination of distance constraints in proteins.
HostingRepository	PRIDE
AnnounceDate	2020-02-28
AnnouncementXML	Submission_2020-02-28_08:47:28.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jan Fiala
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	iodoacetamide derivatized residue
Instrument	solariX

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-28 02:38:43	ID requested
⏵ 1	2020-02-28 08:47:29	announced
2	2020-11-06 08:47:58	announced	2020-11-06: Updated project metadata.
3	2024-10-22 05:00:08	announced	2024-10-22: Updated project metadata.

Fiala J, Kuka, č, ka Z, Nov, á, k P, Influence of cross-linker polarity on selectivity towards lysine side chains. J Proteomics, 218():103716(2020) [pubmed]

submitter keyword: Structural mass spectrometry

protein structure

chemical cross-linking

disuccinimidyl glutarate

bis(sulfosuccinimidyl) glutarate

bovine serum albumin

Petr Novák
contact affiliation	Institute of Microbiology of the Czech Academy of Sciences, Vestec, Czech Republic
contact email	pnovak@biomed.cas.cz
lab head
Jan Fiala
contact affiliation	1 Institute of Microbiology of the Czech Academy of Sciences, Vestec, Czech Republic 2 Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic
contact email	jan.fiala@biomed.cas.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/02/PXD017299

PRIDE project URI

• PRIDE
  1. PXD017299
     1. Label: PRIDE project
     2. Name: Influence of cross-linker polarity on selectivity towards lysine side chains