PXD017298-1

PXD017298 is an original dataset announced via ProteomeXchange.

Title	Identification of gene fusion events in Mycobacterium tuberculosis that encode novel chimeric proteins
Description	Mycobacterium tuberculosis is a facultative intracellular pathogen, responsible for causing tuberculosis. The harsh environment in which M. tuberculosis survives requires this pathogen to maintain an evolutionary advantage. However, the apparent absence of horizontal gene transfer in M. tuberculosis imposes restrictions in the ways by which evolution can occur. Large scale changes in the genome can be introduced through genome reduction, recombination events and structural variation. Here, we identify a functional chimeric protein in the ppe38-71 locus, the absence of which is known to have an impact on protein secretion and virulence. To examine whether this approach was used more often by this pathogen we further develop software that detects potential gene fusion events from multigene deletions using whole-genome sequencing data. With this software we could identify a number of other putative gene-fusion events within the genomes of M. tuberculosis isolates. We were able to demonstrate the expression of one of these gene fusions at the protein level using mass spectrometry. Therefore, gene fusions may provide an additional means of evolution for M. tuberculosis in its natural environment whereby novel proteins and functions can arise.
HostingRepository	PRIDE
AnnounceDate	2020-05-12
AnnouncementXML	Submission_2020-05-12_08:03:26.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Samantha Sampson
SpeciesList	scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: 83332;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-28 02:38:26	ID requested
⏵ 1	2020-05-12 08:03:26	announced
2	2024-10-22 05:03:32	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Protoemics, Gene fusions, Genomics

Samantha Sampson
contact affiliation	DST-NRF Center for Excellence in Biomedical TB research south African Medical Research Council Center for Tuberculosis Research Division of Molecular Biology and Human Genetics Department of Biomedical Science Faculty of Medicine and Health Science Stellenbosch University Cape Town South Africa
contact email	ssampson@sun.ac.za
lab head
Samantha Sampson
contact affiliation	Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town
contact email	ssampson@sun.ac.za
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017298
     1. Label: PRIDE project
     2. Name: Identification of gene fusion events in Mycobacterium tuberculosis that encode novel chimeric proteins