PXD017291-1

PXD017291 is an original dataset announced via ProteomeXchange.

Title	Aggregation and disaggregation features of the human proteome
Description	The aim of the project was to quantify protein aggregation and disaggregation in human cells after transient non-lethal heat shock and during recovery. In addition, the non-aggregating proteins were analyzed by two-dimensional proteome profiling to detect changes in thermal stability upon heat shock. For aggregation/disaggregation study, K562 cells were grown in light SILAC medium which was changed to heavy medium 90 minutes before heat treatment (10 minutes at 44C). After heat shock, cells were let to recover at 37C. Samples were collected before and after the heat shock as well as on multiple time points during recovery (one, two, three and five hours). Protein intensities from soluble fraction (extracted with mild detergent - NP40) was compared to a control samples that on parallel were treated with mock shock (10 minutes at +37C). Samples were labelled with TMT labels and pooled. The medium switch prior to heat shock also allowed to monitor changes in protein synthesis caused by the heat shock. For control, an analysis of total protein amount (extracted with strong detergent - SDS) was conducted. For two-dimensional proteome profiling, aliquots of heat shocked and mock shocked samples were exposed to a temperature gradient (from 37.0C to 66.3C). Samples from two adjacent temperatures were labelled with TMT and pooled. Proteins from soluble fractions were quantified and heat shock-induced changes in thermal stability were analyzed.
HostingRepository	PRIDE
AnnounceDate	2020-09-22
AnnouncementXML	Submission_2020-09-22_03:34:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tomi Määttä
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	isotope labeled residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-27 03:46:56	ID requested
⏵ 1	2020-09-22 03:34:06	announced
2	2020-10-30 06:16:31	announced	2020-10-30: Updated publication reference for PubMed record(s): 33022891.
3	2024-10-22 05:12:42	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: human, K562, heat shock, protein quality control, aggregation, disaggregation, thermal proteome profiling, thermal stability, SILAC, TMT, hyperplexing, Q Exactive Plus Orbitrap, Orbitrap Fusion Lumos, isobarQuant, LC-MSMS

Mikhail M Savitski
contact affiliation	European Molecular Biology Laboratory
contact email	mikhail.savitski@embl.de
lab head
Tomi Määttä
contact affiliation	European Molecular Biology Laboratory
contact email	tomi.maatta@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017291

PRIDE project URI

• PRIDE
  1. PXD017291
     1. Label: PRIDE project
     2. Name: Aggregation and disaggregation features of the human proteome