PXD017285 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Exposure of THP1 cells to plasma containing or lacking cfDNA |
Description | We performed all stimulatory experiments using THP1 cell line as a representative of primary human monocytes to show fundamental role of the cfDNA in healthy organisms. The experiments were conducted in duplicates using plasma containing cfDNA (NP) and the reference one with cfDNA removed by DNase (TP) to recognize unequivocally the effect of plasma cfDNA on transcriptome and proteome of monocytes. We used native human plasma samples obtained from healthy volunteers with no animal serum addition to cultivation medium in order to avoid the presence of uncharacterized animal cfDNA in the experiments. |
HostingRepository | PRIDE |
AnnounceDate | 2021-05-19 |
AnnouncementXML | Submission_2021-05-19_01:22:38.154.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD017285 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Karel Harant |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ FT |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-01-27 01:26:41 | ID requested | |
⏵ 1 | 2021-05-19 01:22:39 | announced | |
Publication List
Korabecna M, Zinkova A, Brynychova I, Chylikova B, Prikryl P, Sedova L, Neuzil P, Seda O, Cell-free DNA in plasma as an essential immune system regulator. Sci Rep, 10(1):17478(2020) [pubmed] |
Keyword List
submitter keyword: THP1 cells, plasma, cfDNA |
Contact List
Marie Korabecna |
contact affiliation | Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic |
contact email | Marie.Korabecna@lf1.cuni.cz |
lab head | |
Karel Harant |
contact affiliation | Charles University |
contact email | harant@natur.cuni.cz |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD017285 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017285
- Label: PRIDE project
- Name: Exposure of THP1 cells to plasma containing or lacking cfDNA