⮝ Full datasets listing
PXD017281-1
PXD017281 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Parallel reaction monitoring assay development for multiple sclerosis biomarkers in cerebrospinal fluid. |
| Description | We have developed and tested parallel reaction monitoring (PRM) assays for 25 proteins highly relevant as cerebrospinal fluid (CSF) biomarkers for multiple sclerosis, representing various biological processes affected in the disease. The proteins were represented by 72 peptides selected according to relevant guidelines and available literature. Stability testing revealed 64 peptides with low intra- and inter-day variations, with 44 also being stably digested after 16 hours of trypsin digestion, and 37 furthermore showing a significant difference between multiple sclerosis and controls, thereby confirming literature findings. Calibration curves were developed using spike-in of synthetic light and heavy peptides in rat plasma. |
| HostingRepository | PanoramaPublic |
| AnnounceDate | 2021-05-20 |
| AnnouncementXML | Submission_2021-05-20_10:27:43.543.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Astrid Guldbrandsen |
| SpeciesList | scientific name: Rattus norvegicus; NCBI TaxID: 10116; scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4) |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2020-01-24 11:26:26 | ID requested | |
| ⏵ 1 | 2021-05-20 10:27:44 | announced |
Publication List
| Guldbrandsen A, Lereim RR, Jacobsen M, Garberg H, Kroksveen AC, Barsnes H, Berven FS, Development of robust targeted proteomics assays for cerebrospinal fluid biomarkers in multiple sclerosis. Clin Proteomics, 17():33(2020) [pubmed] |
Keyword List
| submitter keyword: parallel reaction monitoring, cerebrospinal fluid, multiple sclerosis, neurological diseases, proteomics, biomarker |
Contact List
| Frode Berven | |
|---|---|
| contact affiliation | Proteomics Unit (PROBE), Department of Biomedicine, University of Bergen, Norway |
| contact email | fberven76@gmail.com |
| lab head | |
| Astrid Guldbrandsen | |
| contact affiliation | Proteomics Unit (PROBE), Department of Biomedicine, University of Bergen, Norway |
| contact email | astrid.guldbrandsen@uib.no |
| dataset submitter | |
Full Dataset Link List
| Panorama Public dataset URI |
