PXD017251-1
PXD017251 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Deep Characterization of the IFN-I/III-Stimulated Proteome in HBV-Transfected or Native HepG2 Cells |
Description | To expand knowledge of the effects of interferon at the proteomic level, we treated HepG2 cells with IFN-alpha and IFN-lambda for 24 hours. HepG2.2.15 cells, a model for HBV infection, were also examined versus controls. MTT assays showed that optimized IFN levels (100 ng/ml) did not induce apoptosis relative to untreated controls. Including controls, more than 6,000 proteins were identified. Five replicates each of IFN-alpha treatment, IFN-lambda treatment, and control were performed, allowing confident identification of differentially expressed proteins. While a number of publications suggest that no interferon effect is evident upon HBV infection, our own results strongly suggest otherwise. Differential alterations of the proteasome were noted when comparing HBV infection against IFN-treatment. We also note that differential effects upon IFN treatment significantly overlapped with transcriptomic datasets when upregulation was examined. However, proteins downregulated upon IFN treatment show little overlap with these transcriptomic datasets. |
HostingRepository | PRIDE |
AnnounceDate | 2020-06-26 |
AnnouncementXML | Submission_2020-06-26_05:55:49.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Poorichaya Somparn |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: HBV genotype D; NCBI TaxID: 489483; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-01-22 03:41:34 | ID requested | |
⏵ 1 | 2020-06-26 05:55:50 | announced | |
2 | 2020-06-29 00:56:08 | announced | 2020-06-29: Updated publication reference for DOI(s): null. |
3 | 2020-07-22 00:05:22 | announced | 2020-06-29: Updated publication reference for DOI(s): null. 2020-07-22: Updated publication reference for PubMed record(s): 32685848. 2020-07-22: Updated publication reference for DOI(s): null. |
4 | 2023-11-14 09:01:03 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Hepatitis B virus, Interferon alpha, Interferon lambda3, Quantitative proteomics |
Contact List
Trairak Pisitkun | |
---|---|
contact affiliation | Center of Excellence in Systems Biology, Research Affairs, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand |
contact email | trairak@gmail.com |
lab head | |
Poorichaya Somparn | |
contact affiliation | Chulalongkorn university |
contact email | poorichaya.s@chula.ac.th |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD017251 |
PRIDE project URI |
Repository Record List
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