PXD017196-2

PXD017196 is an original dataset announced via ProteomeXchange.

Title	The dissociation of RQT complex on ribosome
Description	Ribosome-associated quality control (RQC) represents a rescue pathway in eukaryotic cells triggered upon translational stalling. Collided ribosomes are recognized for subsequent dissociation followed by targeting of the faulty nascent peptides for degradation. However, endogenous RQC inducing sequences and the mechanism underlying the ubiquitin-dependent ribosome dissociation remain poorly understood. Here, we identified the SDD1 mRNA from S. cerevisiae as an endogenous RQC substrate and reveal its mRNA and nascent peptide dependent stalling mechanism by mutational and cryo-EM analyses. In vitro translation of SDD1 mRNA enabled the reconstitution of Hel2-dependent poly-ubiquitination of colliding di- and preferentially tri-ribosomes. Their distinct architecture is visualized by cryo-EM and provides the structural basis for more efficient recognition by Hel2 over disomes. Subsequently, the Slh1/Rqt2 helicase subunit of the RQC trigger (RQT) complex preferentially dissociates the first stalled poly-ubiquitinated ribosome in an ATP dependent manner. Together, these findings provide fundamental mechanistic insights into RQC and its physiological role in dealing with endogenous substrates to maintain cellular protein homeostasis.
HostingRepository	PRIDE
AnnounceDate	2020-02-24
AnnouncementXML	Submission_2020-05-18_07:00:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Akinori Endo
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	ubiquitinylated lysine; methylthiolated residue; monohydroxylated residue; acetylated residue; deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-20 02:57:02	ID requested
1	2020-02-24 01:47:24	announced
⏵ 2	2020-05-18 07:00:17	announced	2020-05-18: Updated publication reference for PubMed record(s): 32203490.
3	2024-10-22 04:59:18	announced	2024-10-22: Updated project metadata.

Matsuo Y, Tesina P, Nakajima S, Mizuno M, Endo A, Buschauer R, Cheng J, Shounai O, Ikeuchi K, Saeki Y, Becker T, Beckmann R, Inada T, RQT complex dissociates ribosomes collided on endogenous RQC substrate SDD1. Nat Struct Mol Biol, 27(4):323-332(2020) [pubmed]

submitter keyword: Yeast, in vitro reaction, ribosome

Toshifumi Inada
contact affiliation	Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578, Japan
contact email	tinada@m.tohoku.ac.jp
lab head
Akinori Endo
contact affiliation	Tokyo Metropolitan Institute of Medical Science
contact email	endo-ak@igakuken.or.jp
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017196
     1. Label: PRIDE project
     2. Name: The dissociation of RQT complex on ribosome