PXD017187 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | OsNSUN2-mediated 5-methylcytidine mRNA modification enhances rice adaptation to high-temperature |
Description | Heat stress is one of the extreme weather disasters potently decreasing cereal plant production. Recent studies have demonstrated that protein degradation and rRNA homeostasis as well as transcription factors are involved in thermoresponse in plants. However, how 5-methylcytosine (m5C) RNA modification contribute to heat stress response in plant remains largely unknown. Herein, we identified OsNSUN2 as the mRNA m5C methyltransferase in rice. The osnsun2 mutants specifically displayed severe phenotypes of temperature- and light-dependent lesion mimic and heat stress hypersensitivity. Heat stress enhanced mRNAs m5C methylation involving photosynthesis and detoxification systems, such as β-OsLCY, OsHO2, OsPAL1, and OsGLYI4, in an OsNSUN2-dependent manner, which then increased the protein synthesis. Consistently, the photosystem of the osnsun2 mutants was vulnerable to high ambient temperature and failed to be repaired under tolerable heat stress. Furthermore, OsNSUN2 mutation reduced photosynthesis efficiency and accumulated excessive reactive oxygen species upon heat treatment. Our findings demonstrate an important mechanism of mRNA m5C-dependent heat acclimation in rice. |
HostingRepository | PRIDE |
AnnounceDate | 2020-04-15 |
AnnouncementXML | Submission_2020-04-15_02:52:25.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Chunchun GAO |
SpeciesList | scientific name: Rice(O. sativa); NCBI TaxID: 4530; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-01-20 00:21:14 | ID requested | |
⏵ 1 | 2020-04-15 02:52:27 | announced | |
Publication List
Tang Y, Gao CC, Gao Y, Yang Y, Shi B, Yu JL, Lyu C, Sun BF, Wang HL, Xu Y, Yang YG, Chong K, OsNSUN2-Mediated 5-Methylcytosine mRNA Modification Enhances Rice Adaptation to High Temperature. Dev Cell, 53(3):272-286.e7(2020) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: NSUN, rice,Oryza Sativa, heat stress, m5C methylation, RNA modification, methyltransferase, photosynthesis, ROS |
Contact List
Kang Chong |
contact affiliation | Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China |
contact email | chongk@ibcas.ac.cn |
lab head | |
Chunchun GAO |
contact affiliation | Beijing Institute of Genomics |
contact email | gaochunchun@big.ac.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017187
- Label: PRIDE project
- Name: OsNSUN2-mediated 5-methylcytidine mRNA modification enhances rice adaptation to high-temperature