PXD017172-1

PXD017172 is an original dataset announced via ProteomeXchange.

Title	Identification of osteolineage cell-derived extracellular vesicle cargo implicated in hematopoietic support
Description	Osteolineage cell-derived extracellular vesicles (EVs) play a regulatory role in hematopoiesis and have been shown to promote the ex vivo expansion of human hematopoietic stem and progenitor cells (HSPCs). Here, we demonstrate that EVs from different human osteolineage sources do not have the same HSPC expansion promoting potential. Comparison of stimulatory and non-stimulatory osteolineage EVs by next-generation sequencing and mass spectrometry analyses revealed distinct microRNA and protein signatures identifying EV-derived candidate regulators of ex vivo HSPC expansion. Accordingly, the treatment of umbilical cord blood-derived CD34+ HSPCs with stimulatory EVs altered HSPC transcriptome, including genes with known roles in cell proliferation. An integrative bioinformatics approach, which connects the HSPC gene expression data with the candidate cargo in stimulatory EVs, delineated the potentially targeted biological functions and pathways during hematopoietic cell expansion and development. In conclusion, our study gives novel insights into the complex biological role of EVs in osteolineage cell-HSPC crosstalk and promotes the utility of EVs and their cargo as therapeutic agents in regenerative medicine.
HostingRepository	PRIDE
AnnounceDate	2020-02-28
AnnouncementXML	Submission_2020-02-28_00:08:21.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Floris van Alphen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-17 06:02:12	ID requested
⏵ 1	2020-02-28 00:08:23	announced
2	2024-10-22 04:59:13	announced	2024-10-22: Updated project metadata.

Morhayim J, Ghebes CA, Erkeland SJ, Ter Borg MND, Hoogenboezem RM, Bindels EMJ, van Alphen FPJ, Kassem M, van Wijnen AJ, Cornelissen JJ, van Leeuwen JP, van der Eerden BCJ, Voermans C, van de Peppel J, Braakman E, Identification of osteolineage cell-derived extracellular vesicle cargo implicated in hematopoietic support. FASEB J, 34(4):5435-5452(2020) [pubmed]

submitter keyword: Intercellular communication, hematopoietic niche, hematopoietic expansion, bone

Prof. Dr. Sander Meijer
contact affiliation	Department of Molecular and Cellular Hemostasis, Sanquin Research, Amsterdam, the Netherlands.
contact email	S.Meijer@sanauin.nl
lab head
Floris van Alphen
contact affiliation	Sanquin Research
contact email	F.vanalphen@sanquin.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/02/PXD017172

PRIDE project URI

• PRIDE
  1. PXD017172
     1. Label: PRIDE project
     2. Name: Identification of osteolineage cell-derived extracellular vesicle cargo implicated in hematopoietic support