PXD017171 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comprehensive functional analysis of Escherichia coli ribosomal RNA methyltransferases |
Description | We analyzed total proteome of three methyltransferase gene knockouts strains of Escherichia coli: ΔrsmF (JW5301), ΔrlmC (JW2756), ΔrlmE (JW3146) from Keio collection and wild type (WT). Proteins were assessed using IDA approach (i.e. Information Dependent Acquisition) and SWATH (Data-Independent Acquisition) on Sciex TripleTof 5600+ mass‐spectrometer with a NanoSpray III ion source (ABSciex, Canada) coupled to a NanoLC Ultra 2D+ nano‐HPLC system (Eksigent). Dataset covers 16 samples. |
HostingRepository | PRIDE |
AnnounceDate | 2020-01-21 |
AnnouncementXML | Submission_2020-01-21_02:00:20.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Olga Bukato |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-01-17 06:02:09 | ID requested | |
⏵ 1 | 2020-01-21 02:00:21 | announced | |
2 | 2024-10-22 04:59:26 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Escherichia coli, LC-MSMS, IDA |
Contact List
Petr Sergiev |
contact affiliation | Lomonosov Moscow State University, Moscow, Russia |
contact email | petya@belozersky.msu.ru |
lab head | |
Olga Bukato |
contact affiliation | Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency |
contact email | olya.bukato@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017171
- Label: PRIDE project
- Name: Comprehensive functional analysis of Escherichia coli ribosomal RNA methyltransferases