<<< Full experiment listing

PXD017171-1

PXD017171 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComprehensive functional analysis of Escherichia coli ribosomal RNA methyltransferases
DescriptionWe analyzed total proteome of three methyltransferase gene knockouts strains of Escherichia coli: ΔrsmF (JW5301), ΔrlmC (JW2756), ΔrlmE (JW3146) from Keio collection and wild type (WT). Proteins were assessed using IDA approach (i.e. Information Dependent Acquisition) and SWATH (Data-Independent Acquisition) on Sciex TripleTof 5600+ mass‐spectrometer with a NanoSpray III ion source (ABSciex, Canada) coupled to a NanoLC Ultra 2D+ nano‐HPLC system (Eksigent). Dataset covers 16 samples.
HostingRepositoryPRIDE
AnnounceDate2020-01-21
AnnouncementXMLSubmission_2020-01-21_02:00:20.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterOlga Bukato
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListNo PTMs are included in the dataset
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-01-17 06:02:09ID requested
12020-01-21 02:00:21announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Escherichia coli, LC-MSMS, IDA
Contact List
Petr Sergiev
contact affiliationLomonosov Moscow State University, Moscow, Russia
contact emailpetya@belozersky.msu.ru
lab head
Olga Bukato
contact affiliationFederal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
contact emailolya.bukato@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD017171
PRIDE project URI
Repository Record List
[ + ]