PXD017050-3

PXD017050 is an original dataset announced via ProteomeXchange.

Title	A quantitative tissue-specific landscape of protein redox regulation during aging
Description	Mammalian tissues engage in specialized physiology that is regulated by post-translational protein modification. A major mode of protein regulation is initiated by reactive oxygen species (ROS) that reversibly modify protein cysteine residues. ROS regulate a myriad of biological processes and ROS dysregulation has long been implicated in age-related dysfunction. However, the protein targets of ROS modification that underlie tissue-specific physiology in vivo are largely unknown. Here we develop a mass spectrometric technology for the first comprehensive and quantitative mapping of the mouse cysteine redox proteome in vivo. We report the cysteine redox landscape across 10 tissues in young and old mice and establish several unexpected and fundamental paradigms of redox signaling. We define and validate cysteine redox networks within each tissue that are highly tissue-selective and underlie tissue-specific biology. We determine a common mechanism for encoding cysteine redox sensitivity by local electrostatic gating. Finally, we comprehensively identify redox-modified disease networks that remodel in aged mice, providing a systemic molecular basis for the longstanding proposed links between redox dysregulation and tissue aging. We provide the Oximouse compendium as a framework for understanding mechanisms of redox regulation in physiology and aging.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:00:01.272.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haopeng Xiao
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-10 01:26:42	ID requested
1	2020-02-27 02:01:50	announced
2	2020-05-27 04:14:08	announced	2020-05-27: Updated publication reference for PubMed record(s): 32109415.
⏵ 3	2024-10-22 05:00:07	announced	2024-10-22: Updated project metadata.

Xiao H, Jedrychowski MP, Schweppe DK, Huttlin EL, Yu Q, Heppner DE, Li J, Long J, Mills EL, Szpyt J, He Z, Du G, Garrity R, Reddy A, Vaites LP, Paulo JA, Zhang T, Gray NS, Gygi SP, Chouchani ET, A Quantitative Tissue-Specific Landscape of Protein Redox Regulation during Aging. Cell, 180(5):968-983.e24(2020) [pubmed]

10.1016/j.cell.2020.02.012;

curator keyword: Biological

submitter keyword: CPT, redox, aging, cysteine proteomics

Edward Chouchani
contact affiliation	1) Department of Cancer Biology, Dana–Farber Cancer Institute, Boston, MA, USA. 2) Department of Cell Biology, Harvard Medical School, Boston, MA, USA.
contact email	edwardt_chouchani@dfci.harvard.edu
lab head
Haopeng Xiao
contact affiliation	Dana-Farber Cancer Institute/Harvard Medical School
contact email	haopeng_xiao@dfci.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/02/PXD017050

PRIDE project URI

• PRIDE
  1. PXD017050
     1. Label: PRIDE project
     2. Name: A quantitative tissue-specific landscape of protein redox regulation during aging