PXD017047 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Isolation and whole protein characterization of species A and B bovine rotaviruses from Chinese calves. |
Description | Rotaviruses (RVs) account for severe diarrhea in children and young animals globally. In the current study, the fecal samples of diarrheic calves from a beef farm in Inner Mongolia were screened for RVA by ELISA and RT- PCR, followed by culture of three positive RVA samples in the MA-104 cell line. After 10 blind passages, cytopathic effects (CPE) appeared as detachment, granulation, and clustering of the inoculated cells. The virus isolates were identified by RT-PCR (VP6 gene RVA) and ESI-LC-MS/MS for whole protein sequencing. The protein sequences demonstrated the presence of two strains from species A rotavirus and one RVB strain; RVA/Cow-tc/ CHN/35333/2019/G6P[5] was mixed with one RVB strain (RVB/Cow-tc/CHN/35334/2019/G5P[3]) in two samples, and RVA/Cow-tc/CHN/10927/2019/G8P[7] was found in one sample. They are of genotype constellations (G6-P[5]-I2-R2-C2-M2-A3-N2-T6-E2-H3), (G8-P[7]-I5-R1-C1- M2-A1-N1-T1-E1-H1), and (G5-P[3]-I3-R5- C5-A5-N4-H5), respectively. Besides, phylogenetic analysis of the obtained sequences demonstrated viral evolution. |
HostingRepository | PRIDE |
AnnounceDate | 2021-01-18 |
AnnouncementXML | Submission_2021-01-17_23:59:57.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Gehad Elkady |
SpeciesList | scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; |
ModificationList | S-pyridylethyl-L-cysteine |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-01-10 01:22:39 | ID requested | |
⏵ 1 | 2021-01-17 23:59:58 | announced | |
Publication List
Elkady G, Zhu J, Peng Q, Chen M, Liu X, Chen Y, Hu C, Chen H, Guo A, Isolation and whole protein characterization of species A and B bovine rotaviruses from Chinese calves. Infect Genet Evol, 89():104715(2021) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Bovine Rotavirus, BRA,BRB, Protein typing, ESl-LC- MS/MS |
Contact List
Aizhen Guo |
contact affiliation | College of Veterinary Medicine, The state Key Laboratory of Agricultural Microbiology, Cooperative Innovation Center of Substantial Pig Production, Wuhan, 433070, China |
contact email | aizhen@mail.hzau.edu.cn |
lab head | |
Gehad Elkady |
contact affiliation | College of Veterinary Medicine, HZAU |
contact email | 2307957696@qq.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017047
- Label: PRIDE project
- Name: Isolation and whole protein characterization of species A and B bovine rotaviruses from Chinese calves.