PXD017046 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of NPGPx-interacting proteins in activated T cells |
Description | The tyrosine kinase, ΞΆ-chain-associated protein of 70 kDa (ZAP70), is essential for T cell development and activation. However, it remains elusive whether a direct redox regulation affects ZAP70 activity upon TCR stimulation. Here, we showed that deficiency of non-selenocysteine containing phospholipid hydroperoxide glutathione peroxidase (NPGPx), a redox sensor, resulted in T cell hyperproliferation and elevated cytokine productions. Through proteomic approaches, ZAP70 is identified as the key interacting protein of NPGPx through disulfide bonding. Here we utilized the MS/MS spectra to identify the cysteine residues in ZAP70 for NPGPx-mediated regulation. Combined molecular approaches, our results elucidate a delicate redox mechanism that NPGPx serves as a modulator to curb ZAP70 functions in maintaining T cell homeostasis. |
HostingRepository | PRIDE |
AnnounceDate | 2021-01-25 |
AnnouncementXML | Submission_2021-01-24_23:01:02.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Pang-Hung Hsu |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-01-10 00:35:13 | ID requested | |
⏵ 1 | 2021-01-24 23:01:02 | announced | |
Publication List
Su FY, Huang SC, Wei PC, Hsu PH, Li JP, Su LW, Hsieh YL, Hu CM, Hsu JL, Yang CY, Chung CY, Shew JY, Lan JL, Sytwu HK, Lee EY, Lee WH, Redox sensor NPGPx restrains ZAP70 activity and modulates T cell homeostasis. Free Radic Biol Med, 165():368-384(2021) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: NPGPx, ZAP70, T cell activation |
Contact List
Pang-Hung Hsu |
contact affiliation | National Taiwan Ocean University |
contact email | phsu@ntou.edu.tw |
lab head | |
Pang-Hung Hsu |
contact affiliation | National Taiwan Ocean University |
contact email | phsu@ntou.edu.tw |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD017046 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017046
- Label: PRIDE project
- Name: Identification of NPGPx-interacting proteins in activated T cells