PXD017004-2

PXD017004 is an original dataset announced via ProteomeXchange.

Title	Phosphorylation regulates secretion of Mycobacterium tuberculosisvirulence mediators ESAT6 and CFP10
Description	Virulence effectors secreted by Mycobacterium tuberculosis help subvert host immune mechanisms and therefore are critical for establishment of infection and pathogenesis. However, knowledge in terms of signaling mechanisms thatmodulate the secretion of virulence factors is sparse. Here using high-throughput mass-spectrometric analysis of mycobacterial secretome,phospho-proteome and phospho-secretome combined with empirical validations, we show an unprecedented regulation of mycobacterial secretion via protein phosphorylation. The intricate relationship between phosphorylation and secretion was revealed by system-level analysis of PPI network superimposed with secretome and phospho-proteome profiles. This study establishes phosphorylation as a keydeterminant of secretion of virulence factors and unveils as yet unexplored targets for therapeutic interventions.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:03:23.186.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	basanti malakar
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: 83332;
ModificationList	phosphorylated residue
Instrument	LTQ Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-08 01:20:01	ID requested
1	2023-09-18 05:27:10	announced
⏵ 2	2024-10-22 06:03:23	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: CFP10,Mycobacterium, Secretion, ESAT6, Phosphorylation

Vinay Kumar Nandicoori
contact affiliation	Signal Transduction Lab 1, National Institute of Immunology, India
contact email	vinaykn@nii.ac.in
lab head
basanti malakar
contact affiliation	National Institute of Immunology
contact email	basantimalakar@nii.ac.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017004
     1. Label: PRIDE project
     2. Name: Phosphorylation regulates secretion of Mycobacterium tuberculosisvirulence mediators ESAT6 and CFP10