PXD016985-1

PXD016985 is an original dataset announced via ProteomeXchange.

Title	Analysis of Brain Protein Stability Changes in Mouse Models of Normal Aging and a Synucleinopathy Reveals Age- and Disease-Related Differences
Description	The lack of appropriate protein biomarkers makes the early detection of Parkinson’s disease (PD) challenging, and it thwarts efforts to develop drug therapies for this incurable disease. Thus, new approaches are needed for biomarker discovery and for better understanding the molecular basis of PD progression. Here, we utilize proteome-wide measurements of protein folding stability to characterize the progression of PD in a mouse model of the disease in which the human α-synuclein protein with an A53T mutation was overexpressed. Using the Stability of Proteins from Rates of Oxidation (SPROX) technique, the thermodynamic stabilities of proteins in brain tissue cell lysates from Huα-Syn(A53T) transgenic mice were profiled at three time points including at 1 month (n=9), at 6 months (n=7), and at the time (between 9 and 16 months) a mouse became Symptomatic (n=8).
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:35:08.621.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Renze Ma
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	methylthiolated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; deamidated residue
Instrument	Q Exactive HF; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-07 04:49:43	ID requested
⏵ 1	2024-10-07 10:35:09	announced

10.1021/acs.jproteome.1c00653;

Ma R, Johnson JHR, Tang Y, Fitzgerald MC, -Synucleinopathy Reveals Age- and Disease-Related Differences. J Proteome Res, 20(11):5156-5168(2021) [pubmed]

curator keyword: Biomedical

submitter keyword: Brain, Mouse,Parkinson's Disease

Michael Fitzgerald
contact affiliation	Professor, Chemistry, Duke University
contact email	michael.c.fitzgerald@duke.edu
lab head
Renze Ma
contact affiliation	Duke University
contact email	renze.ma@duke.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD016985

PRIDE project URI

• PRIDE
  1. PXD016985
     1. Label: PRIDE project
     2. Name: Analysis of Brain Protein Stability Changes in Mouse Models of Normal Aging and a Synucleinopathy Reveals Age- and Disease-Related Differences