PXD016962-1

PXD016962 is an original dataset announced via ProteomeXchange.

Title	Genome-scale transcription-translation mapping reveals features of Zymomonas mobilis transcription units and promoters
Description	Limited functional annotation of the Z. mobilis genome is a current barrier to both basic studies of Z. mobilis and its development as a synthetic-biology chassis. To gain insight, we collected sample-matched multiomics data including RNA-seq, transcription start site sequencing (TSS-seq), termination sequencing (term-seq), ribosome profiling, and label-free shotgun proteomic mass spectrometry across different growth conditions to improve annotation and assign functional sites in the Z. mobilis genome. Proteomics and ribosome profiling informed revisions of protein-coding genes, which included 44 start codon changes and 42 added proteins.
HostingRepository	PRIDE
AnnounceDate	2020-07-10
AnnouncementXML	Submission_2020-07-09_22:39:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jessica Vera
SpeciesList	scientific name: Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821; NCBI TaxID: 264203;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-06 05:17:54	ID requested
⏵ 1	2020-07-09 22:39:58	announced
2	2024-10-22 05:08:44	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Zymomonas mobilis, proteogenomics, shotgun proteomics, multiomics

Robert Landick
contact affiliation	Department of Biochemistry and Department of Bacteriology University of Wisconsin-Madison, Madison, WI 53706 Dept. of Energy Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI 53726
contact email	rlandick@wisc.edu
lab head
Jessica Vera
contact affiliation	Great Lakes Bioenergy Research Center
contact email	jmvera@wisc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD016962

PRIDE project URI

• PRIDE
  1. PXD016962
     1. Label: PRIDE project
     2. Name: Genome-scale transcription-translation mapping reveals features of Zymomonas mobilis transcription units and promoters