PXD016894-2
PXD016894 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Comparative phosphoproteomic analysis of raf36raf22 in Arabidopsis |
| Description | A phythormone abscisic acid (ABA) has a major role in abiotic stress responses in plants, and subclass III SNF1-related protein kinase 2 (SnRK2) mediates ABA signaling. In this study, we identified Raf36, a group C Raf-like protein kinase in Arabidopsis, as an interacting protein with SnRK2. A series of reverse genetic and biochemical analyses revealed that Raf36 negatively regulates ABA responses and is directly phosphorylated by SnRK2s. In addition, we found that Raf22, another C-type Raf-like kinase, functions partially redundantly with Raf36 to regulate ABA responses. Comparative phosphoproteomic analysis using Arabidopsis wild-type and raf22raf36-1 plants indicate proteins that are phosphorylated downstream of Raf36 and Raf22 in planta. Together, these results reveal a novel subsection of ABA-responsive phosphosignaling pathways branching from SnRK2. |
| HostingRepository | jPOST |
| AnnounceDate | 2020-12-22 |
| AnnouncementXML | Submission_2021-02-04_16:58:33.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Shinnosuke ISHIKAWA |
| SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine |
| Instrument | TripleTOF 5600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2019-12-22 03:00:02 | ID requested | |
| 1 | 2020-12-21 07:00:04 | announced | |
| ⏵ 2 | 2021-02-04 16:58:34 | announced | 2021-02-04: Updated FTP location. |
| 3 | 2022-09-18 02:46:21 | announced | 2022-09-18: Updated FTP location. |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: phosphoproteome, Raf, Arabidopsis, abscisic acid |
Contact List
| Shinnosuke Ishikawa | |
|---|---|
| lab head | |
| Shinnosuke ISHIKAWA | |
| contact affiliation | Tokyo University of Agriculture and Technology |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.biosciencedbc.jp/archive/jpostrepos/JPST000630 |
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