PXD016875 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | An Anti-Cancer Gold(III)-Activated Porphyrin Scaffold that Covalently Modifies Protein Cysteine Thiols |
Description | Cysteine thiols of many cancer-associated proteins are attractive targets of anti-cancer agents. Herein, we unequivocally demonstrate a distinct thiol-targeting property of gold(III) mesoporphyrin IX dimethyl ester (AuMesoIX) and its anti-cancer activities. While the binding of cysteine thiols with metal complexes usually occurs via M–S bond formation, AuMesoIX is unique in that the meso-carbon atom of the porphyrin ring is activated by the gold(III) ion to undergo nucleophilic aromatic substitution with thiols. AuMesoIX was shown to modify reactive cysteine residues and inhibit the activities of anti-cancer protein targets including thioredoxin, peroxiredoxin, and deubiquitinases. Treatment of cancer cells with AuMesoIX resulted in the formation of gold-bound sulfur-rich protein aggregates, oxidative stress-mediated cytotoxicity, and accumulation of ubiquitinated proteins. Importantly, AuMesoIX exhibited effective anti-tumor activity in mice. Our study has uncovered a gold(III)-induced ligand scaffold reactivity for thiol targeting that can be exploited for anti-cancer applications. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:26:38.173.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Di HU |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-12-20 03:19:58 | ID requested | |
1 | 2021-09-08 13:53:10 | announced | |
⏵ 2 | 2024-10-22 05:26:46 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1073/pnas.1915202117; |
Tong KC, Lok CN, Wan PK, Hu D, Fung YME, Chang XY, Huang S, Jiang H, Che CM, An anticancer gold(III)-activated porphyrin scaffold that covalently modifies protein cysteine thiols. Proc Natl Acad Sci U S A, 117(3):1321-1329(2020) [pubmed] |
Keyword List
submitter keyword: Gold • Porphyrin • Ligand Reactivity• Cysteine Thiol Conjugation • Anti-tumor Agents |
Contact List
Chi-Ming Che |
contact affiliation | State Key Laboratory of Synthetic Chemistry and Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, China |
contact email | cmche@hku.hk |
lab head | |
Di HU |
contact affiliation | Department of Chemistry, The University of Hong Kong |
contact email | hudi@hku.hk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016875
- Label: PRIDE project
- Name: An Anti-Cancer Gold(III)-Activated Porphyrin Scaffold that Covalently Modifies Protein Cysteine Thiols