PXD016805-1

PXD016805 is an original dataset announced via ProteomeXchange.

Title	Comparison of the Human Neuronal Proteome upon Japanese Encephalitis or West Nile Virus Infection and potential role of mosquito saliva in neuropathogenesis
Description	Neurotropic flavivirus Japanese encephalitis virus (JEV) and West Nile virus (WNV) are amongst the leading causes of encephalitis. Using label-free quantitative proteomics, we identified proteins differentially expressed upon JEV (gp-3, RP9) or WNV (IS98) infection of human neuroblastoma cells. Both viruses were associated with the up-regulation of immune response (IFIT1/3/5, ISG15, OAS, STAT1, IRF9) and the down-regulation of SSBP2, involved in gene expression, as well as PAM, involved in neuropeptide amidation. Proteins associated to membranes, involved in extracellular matrix organization and collagen metabolism represented major clusters down-regulated by JEV and WNV. Moreover, transcription regulation and mRNA processing clusters were also heavily regulated by both neurotropic flaviviruses. If the proteome of neuroblastoma cells infected by JEV or WNV was significantly modulated in the presence of mosquito saliva, both viruses showed distinct patterns. Mosquito saliva favored the modulation of proteins associated with gene regulation in JEV infected neuroblastoma cells while it was the modulation of proteins associated with protein maturation, signal transduction and ion transporters in the case of WNV infected neuroblastoma cells.
HostingRepository	PRIDE
AnnounceDate	2020-05-14
AnnouncementXML	Submission_2020-05-14_03:51:30.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MARIETTE MATONDO
SpeciesList	scientific name: Culex pipiens pipiens; NCBI TaxID: 38569; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Japanese encephalitis virus; NCBI TaxID: 11072; scientific name: West Nile virus; NCBI TaxID: 11082;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-17 07:48:42	ID requested
⏵ 1	2020-05-14 04:10:23	announced

Besson B, Basset J, Gatellier S, Chabrolles H, Chaze T, Hourdel V, Matondo M, Pardigon N, Choumet V, Comparison of a human neuronal model proteome upon Japanese encephalitis or West Nile Virus infection and potential role of mosquito saliva in neuropathogenesis. PLoS One, 15(5):e0232585(2020) [pubmed]

submitter keyword: West Nile, Japanese encephalitis, Label Free, mosquito

Benoit Besson
contact affiliation	Institut Pasteur
contact email	thibault.chaze@pasteur.fr
lab head
MARIETTE MATONDO
contact affiliation	Pasteur
contact email	mariette.matondo@pasteur.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016805
     1. Label: PRIDE project
     2. Name: Comparison of the Human Neuronal Proteome upon Japanese Encephalitis or West Nile Virus Infection and potential role of mosquito saliva in neuropathogenesis