PXD016793-1

PXD016793 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of rat liver after disulfiram treatment
Description	Obesity has become a global health concern. A molecule that safely prevents and/or treats obesity is urgently needed. Here, we show that long-term diet supplementation of disulfiram (DSF, Antabuse®), an FDA-approved drug, abrogated the adverse impact of an obesogenic diet on insulin responsiveness, while mitigating liver steatosis, cardiovascular remodeling, and pancreatic islet hypertrophy in mice. Additionally, DSF treatment of already obese male and female mice reversed weight gain and alleviated metabolic dysfunctions. Loss of fat tissue and increase in liver fenestrations were also observed in rats on DSF. Transcriptomics and proteomics analyses of mouse and rat livers unveiled comparable signatures, as revealed by pathways associated with lipid and energy metabolism, redox, and detoxification. Mechanistically, markers of hepatic oxidative stress were abrogated in DSF-treated mice through activation of ALDH2 and GST activities in vivo and stimulation of autophagic flux in vitro. Thus, repurposing DSF may represent a new strategy to combat obesity and reverse the associated metabolic disorders in human.
HostingRepository	PRIDE
AnnounceDate	2020-05-15
AnnouncementXML	Submission_2020-05-15_00:00:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mark Larance
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-17 02:03:54	ID requested
⏵ 1	2020-05-15 00:00:59	announced
2	2020-08-11 03:15:15	announced	2020-08-11: Updated publication reference for PubMed record(s): 32714562.

Dataset with its publication pending

submitter keyword: disulfiram, rat, liver, metabolism, high-fat, obesity

Mark Larance
contact affiliation	University of Sydney
contact email	mark.larance@sydney.edu.au
lab head
Mark Larance
contact affiliation	The University of Sydney
contact email	mark.larance@sydney.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016793
     1. Label: PRIDE project
     2. Name: Proteomics analysis of rat liver after disulfiram treatment