PXD016776-1

PXD016776 is an original dataset announced via ProteomeXchange.

Title	Comprehensive chemical proteomics for target deconvolution of the redox active drug auranofin
Description	Chemical proteomics encompasses novel drug target deconvolution methods in which compound modification is not required. Herein we use Thermal Proteome Profiling, Functional Identification of Target by Expression Proteomics and multiplexed redox proteomics for deconvolution of auranofin targets to aid elucidation of its mechanisms of action. Auranofin (Ridaura®) was approved for treatment of rheumatoid arthritis in 1985. Because several clinical trials are currently ongoing to repurpose auranofin for cancer therapy, comprehensive characterization of its targets and effects in cancer cells is important. Together, our chemical proteomics tools confirmed thioredoxin reductase 1 (TXNRD1) as a main auranofin target, with perturbation of oxidoreductase pathways as the top mechanism of drug action. Additional indirect targets included NFKB2 and CHORDC1. Our comprehensive data can be used as a proteomic signature resource for further analyses of the effects of auranofin. Here we also assessed the orthogonality and complementarity of different chemical proteomics methods that can furnish invaluable mechanistic information and thus the approach can facilitate drug discovery efforts in general.
HostingRepository	PRIDE
AnnounceDate	2020-03-19
AnnouncementXML	Submission_2020-03-19_03:21:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Amir Ata Saei
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-16 06:37:03	ID requested
⏵ 1	2020-03-19 03:21:09	announced
2	2024-10-22 05:00:53	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Auranofin, cancer, chemotherapeutic, compound, database, drug, expression, mass spectrometry, proteomics, resource, thioredoxin reductase, NFKB2, CHORDC1, HCT116, A375, RKO

Roman Zubarev
contact affiliation	Department of Medical Biochemistry and Biophysics,Karolinska Institutetl, Stockholm, Sweden
contact email	roman.zubarev@ki.se
lab head
Amir Ata Saei
contact affiliation	Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
contact email	amirata.saei.dibavar@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD016776

PRIDE project URI

• PRIDE
  1. PXD016776
     1. Label: PRIDE project
     2. Name: Comprehensive chemical proteomics for target deconvolution of the redox active drug auranofin