PXD016766-2

PXD016766 is an original dataset announced via ProteomeXchange.

Title	Benchmarking the Orbitrap Tribrid Eclipse for Next Generation Multiplexed Proteomics
Description	The rise of sample multiplexing in quantitative proteomics for the dissection of complex phenotypic comparisons has been advanced by the development of ever more sensitive and robust instrumentation. Here, we evaluated the utility of the Orbitrap Eclipse Tribrid mass spectrometer (advanced quadrupole filter, optimized FTMS scan overhead) and new instrument control software features (Precursor Fit filtering, TurboTMT and Real-time Peptide Search filtering). Multidimensional comparisons of these novel features increased total peptide identifications by 20% for SPS-MS3 methods and 14% for HRMS2 methods. Importantly Real-time Peptide Search filtering enabled a ~2X throughput improvement for quantification. Across the board, these sensitivity increases were attained without sacrificing quantitative accuracy. New hardware and software features enable more efficient characterization in pursuit of comparative whole proteome insights.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:30:47.914.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qing Yu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-16 01:37:14	ID requested
1	2022-01-07 13:36:57	announced
⏵ 2	2024-10-22 05:30:48	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Real-time search, Quantitative proteomics, Eclipse, TurboTMT, Precursor Fit Filter,TMT, Orbitrap, Instrumentation

Steven P Gygi
contact affiliation	Cell biology, Harvard medical school
contact email	steven_gygi@hms.harvard.edu
lab head
Qing Yu
contact affiliation	Harvard Medical School
contact email	qing_yu2@hms.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD016766

PRIDE project URI

• PRIDE
  1. PXD016766
     1. Label: PRIDE project
     2. Name: Benchmarking the Orbitrap Tribrid Eclipse for Next Generation Multiplexed Proteomics