PXD016735-3

PXD016735 is an original dataset announced via ProteomeXchange.

Title	Enhanced interactome capture (eRIC) of steady-state and arsenite stressed HuH7 cell cytoplasmic and nuclear fractions
Description	HuH7 cells were grown under steady-state conditions or exposed 100 µM Na-arsenite for 1h. Subsequently, the cells were irradiated or not with 253 nm UV light (150 mJ/cm^2), processed to nuclear and cytoplasmic fractions, and subjected to eRIC for determining the nuclear and cytoplasmic RNA-binding proteins from the different conditions.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:01:44.190.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frank Stein
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-13 04:16:33	ID requested
1	2020-04-06 05:08:46	announced
2	2020-04-27 00:05:03	announced	2020-04-27: Updated publication reference for PubMed record(s): 32313943.
⏵ 3	2024-10-22 05:01:46	announced	2024-10-22: Updated project metadata.

Backlund M, Stein F, Rettel M, Schwarzl T, Perez-Perri JI, Brosig A, Zhou Y, Neu-Yilik G, Hentze MW, Kulozik AE, Plasticity of nuclear and cytoplasmic stress responses of RNA-binding proteins. Nucleic Acids Res, 48(9):4725-4740(2020) [pubmed]

10.1093/nar/gkaa256;

curator keyword: Biological

submitter keyword: Human, cell culture, arsenite

Andreas E. Kulozik
contact affiliation	Department of Pediatric Oncology, Hematology and Immunology, Heidelberg University, Im Neuenheimer Feld 430, 69120 Heidelberg, Germany
contact email	andreas.kulozik@med.uni-heidelberg.de
lab head
Frank Stein
contact affiliation	EMBL
contact email	frank.stein@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD016735

PRIDE project URI

• PRIDE
  1. PXD016735
     1. Label: PRIDE project
     2. Name: Enhanced interactome capture (eRIC) of steady-state and arsenite stressed HuH7 cell cytoplasmic and nuclear fractions