PXD016726 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Characterization of Macrophage Galactose-type Lectin (MGL) ligands in colorectal cancer cell lines |
Description | Background The Ca2+-dependent C-type lectin receptor Macrophage Galactose-type Lectin (MGL) is highly expressed by tolerogenic dendritic cells (DC) and macrophages. MGL exhibits a high binding specificity for terminal alpha- and beta-linked GalNAc residues found in Tn, sTn and LacdiNAc antigens. These glycan epitopes are often overexpressed in colorectal cancer (CRC), and, as such, MGL can be used to discriminate tumor from the corresponding healthy tissues. Moreover, the high expression of MGL ligands is associated with poor disease-free survival in stage III of CRC tumors. Nonetheless, the glycoproteins expressed by tumor cells that are recognized by MGL have hitherto remained elusive. Methods Using a panel of three CRC cell lines (HCT116, HT29 and LS174T), recapitulating CRC diversity, we performed FACS staining and pull-down assays using a recombinant soluble form of MGL (and a mutant MGL as control) combined with mass spectrometry-based (glyco)proteomics. Results HCT116 and HT29, but not LS174T, are high MGL-binding CRC cell lines. On these cells, the major cell surface binding proteins are receptors (e.g. MET, PTK7, SORL1, PTPRF) and integrins (ITGB1, ITGA3). From these proteins, several N- and/or O-glycopeptides were identified, of which some carried either a LacdiNAc or Tn epitope. |
HostingRepository | PRIDE |
AnnounceDate | 2020-01-09 |
AnnouncementXML | Submission_2020-01-09_07:00:04.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yassene Mohammed |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-12-12 05:37:28 | ID requested | |
⏵ 1 | 2020-01-09 07:00:06 | announced | |
Publication List
Sahasrabudhe NM, Lenos K, van der Horst JC, Rodr, í, guez E, van Vliet SJ, Oncogenic BRAFV600E drives expression of MGL ligands in the colorectal cancer cell line HT29 through N-acetylgalactosamine-transferase 3. Biol Chem, 399(7):649-659(2018) [pubmed] |
Keyword List
submitter keyword: : Glycoproteomics |
C-type lectin |
colorectal cancer |
LacdiNAc |
Tn antigen |
Contact List
Paul J. Hensbergena |
contact affiliation | Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands |
contact email | P.J.Hensbergen@lumc.nl |
lab head | |
Yassene Mohammed |
contact affiliation | Leiden University Medcial Center |
contact email | yassene@proteincentre.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016726
- Label: PRIDE project
- Name: Characterization of Macrophage Galactose-type Lectin (MGL) ligands in colorectal cancer cell lines