PXD016702-1

PXD016702 is an original dataset announced via ProteomeXchange.

Title	Seamount sheath water: years-long retention of deep-ocean water traced by bacterioplankton
Description	Seamounts, often rising hundreds of metres above the surrounding seafloor, obstruct the flow of deep-ocean water. While the resultant entrainment of deep-water by seamounts is predicted from ocean circulation models, its empirical validation has been hampered by the large scale and slow rate of the interaction. To overcome these limitations we use the growth of planktonic bacteria to assess the interaction rate. The selected study site, Tropic Seamount, in the North-Eastern Atlantic represents the majority of isolated seamounts, which do not affect the surface ocean waters. We prove deep-water is entrained by the seamount by measuring 2.3 times higher bacterial concentrations in the seamount-associated or ‘sheath’ water than in deep-ocean water unaffected by seamounts. Genomic analyses of the dominant sheath-water bacteria confirm their planktonic origin, whilst proteomic analyses indicate their slow growth. According to our radiotracer experiments, the doubling time of sheath-water bacterioplankton is 1.5 years. Therefore, for bacterioplankton concentration to reach 2.3 times higher in the ambient seawater, the seamount would need to retain deep-ocean water for more than 3.5 years. We propose that turbulent mixing of the retained sheath-water could stimulate bacterioplankton growth by increasing the cell encounter rate with the ambient dissolved organic molecules. If some of these molecules chelate hydroxides of iron and manganese, bacterioplankton consumption of the organic chelators would result in precipitation of insoluble hydroxides. Hence precipitated hydroxides would form ferromanganese deposits as a result of the bacterioplankton-mediated deep-water seamount interaction.
HostingRepository	PRIDE
AnnounceDate	2020-05-27
AnnouncementXML	Submission_2020-05-26_17:11:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Minia Antelo
SpeciesList	scientific name: unidentified marine bacterioplankton; NCBI TaxID: 13136;
ModificationList	6x(13)C labeled L-lysine; monohydroxylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-11 05:55:10	ID requested
⏵ 1	2020-05-26 17:11:52	announced
2	2024-10-22 05:06:10	announced	2024-10-22: Updated project metadata.

Giljan G, Kamennaya NA, Otto A, Becher D, Ellrott A, Meyer V, Murton BJ, Fuchs BM, Amann RI, Zubkov MV, Bacterioplankton reveal years-long retention of Atlantic deep-ocean water by the Tropic Seamount. Sci Rep, 10(1):4715(2020) [pubmed]

curator keyword: Biological

submitter keyword: Seamount bacterioplankton

ferromanganese encrusted guyot

isotope-tracer bioassay

in situ incubator

flow cytometry

flow sorted cell proteomics

next generation sequencing

Dörte Becher
contact affiliation	Institut für Mikrobiologie Universität Greifswald Felix Hausdorff Str.8 17489 Greifswald Tel.: (+49) 03834 420 5903 Fax.: (+49) 03834 420 5002
contact email	dbecher@uni-greifswald.de
lab head
Minia Antelo
contact affiliation	Institute of Microbiology University of Greifswald
contact email	minia.antelovarela@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD016702

PRIDE project URI

• PRIDE
  1. PXD016702
     1. Label: PRIDE project
     2. Name: Seamount sheath water: years-long retention of deep-ocean water traced by bacterioplankton