PXD016676-1

PXD016676 is an original dataset announced via ProteomeXchange.

Title	Ciliated Neurons Regulate Proteostasis Through Insulin and Calcineurin signaling
Description	Environmental factors contribute to several protein aggregation diseases, however the cause-to-effect relationships remain challenging to study. Here, we genetically abrogate sensory perception in Caenorhabditis elegans to understand the role of neuronal function in governing protein homeostasis. We reveal that defects in neuronal cilia formation reduce signaling via the thermosensory AFD neuron and alleviates protein aggregation. In contrast, ciliary mutants create an aberrant signal emanating from AFD, via the neuropeptide FLP-3 and the insulin-like peptide INS-5, blocking ubiquitin-dependent protein turnover in intestinal cells. Interestingly, lack of the calcineurin A ortholog shifts the balance from insulin towards calcineurin signaling. While both pathways are dispensable during normal sensation, the presence of a neuronal signal triggered by defective cilia primes non-neuronal cells to remodel their protein degradation pathways. In conclusion, our model elucidates the impact of environmental sensation to coordinate the balance between insulin and calcineurin signaling to maintain cellular proteostasis.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-12_14:03:44.692.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexandra Segref
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-10 03:49:44	ID requested
⏵ 1	2022-08-12 14:03:45	announced
2	2023-11-14 07:40:35	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Proteostasis, Ubiquitin, Ciliated Neurons, Insulin signaling, calcineurin signaling, ageing, aggregation

Thorsten Hoppe
contact affiliation	Institute for Genetics and CECAD Research Center, University of Cologne, Joseph-Stelzmann Str. 26, 50931 Cologne, Germany
contact email	thorsten.hoppe@uni-koeln.de
lab head
Alexandra Segref
contact affiliation	University of Cologne
contact email	asegref@uni-koeln.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD016676

PRIDE project URI

• PRIDE
  1. PXD016676
     1. Label: PRIDE project
     2. Name: Ciliated Neurons Regulate Proteostasis Through Insulin and Calcineurin signaling