PXD016666-1

PXD016666 is an original dataset announced via ProteomeXchange.

Title	GroEL interaction and folding of MetF studied by hydrogen/deuterium exchange – mass spectrometry
Description	The cylindrical chaperonin GroEL and its cofactor GroES mediate ATP-dependent protein folding in E. coli by transiently encapsulating non-native substrate in a nano-cage formed by the GroEL ring cavity and the lid-shaped GroES. We analyzed the spontaneous and chaperonin-assisted folding of the essential enzyme 5,10-methylenetetrahydrofolate reductase (MetF) of E. coli, an obligate GroEL/ES substrate. We found that MetF, a homotetramer of 33 kDa subunits with (8) TIM-barrel fold, is unable to fold spontaneously, even in the complete absence of aggregation, and populates a kinetically trapped folding intermediate(s) (MetF-I). GroEL/ES recognized MetF-I and catalyzed its folding with high efficiency, at a half-time of ~4 s and with more than 50% of protein folded in a single round of encapsulation. Analysis by hydrogen/deuterium exchange at peptide resolution showed that the MetF subunit folds to completion in the GroEL/ES nano-cage and binds the co-factor FAD.
HostingRepository	PRIDE
AnnounceDate	2020-03-05
AnnouncementXML	Submission_2020-03-05_05:11:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Balchin
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-09 08:34:26	ID requested
⏵ 1	2020-03-05 05:12:00	announced
2	2020-05-27 05:18:22	announced	2020-05-27: Updated publication reference for PubMed record(s): 32135190.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: hydrogen/deuterium exchange, GroEL, folding, chaperone, chaperonin, MetF

Manajit Hayer-Hartl
contact affiliation	Max Planck Institute of Biochemistry, Department of Cellular Biochemsitry
contact email	mhartl@biochem.mpg.de
lab head
David Balchin
contact affiliation	Max Planck Institute of Biochemistry
contact email	balchin@biochem.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016666
     1. Label: PRIDE project
     2. Name: GroEL interaction and folding of MetF studied by hydrogen/deuterium exchange – mass spectrometry