PXD016645-2

PXD016645 is an original dataset announced via ProteomeXchange.

Title	iTRAQ proteomics reveals the regulatory response to Magnaporthe oryzae in durable resistant vs. susceptible rice genotypes
Description	Here, we used the iTRAQ method to detect the differentially expressed proteins (DEPs) in the durable resistant rice variety Gangyuan8 (GY8) and the susceptible rice variety Lijiangxintuanheigu (LTH) in response to M. oryzae invasion, and then transcriptome sequencing was used to assist analysisA total of 193 and 672 DEPs were specifically identified in GY8 and LTH, respectively, with only 46 similarly expressed DEPs being shared by GY8 and LTH.39 DEPs involved in plant-pathogen interaction, plant hormone signal transduction, fatty acid metabolism and peroxisome biosynthesis were significantly different between compatible interaction (LTH) and incompatible interaction (GY8). Some proteins participated in peroxide signal transduction and biosynthesis was down-regulated in GY8 but up-regulated in LTH. A lot of genes encoding pathogenesis-related gene (PR), such as chitinase and glucanase, were significantly up-regulated at both the transcriptome and proteome levels at 24 hours post-inoculation in GY8, but up-regulated at the transcriptome level and down-regulated at the proteome level in LTH. Our study reveals that the pathogen-associated molecular pattern (PAMP)-triggered immunity defense system may be activated at the transcriptome level but was inhibited at the protein level in susceptible rice varieties after inoculation. The results may facilitate future studies of the molecular mechanisms of rice blast resistance.
HostingRepository	iProX
AnnounceDate	2019-12-09
AnnouncementXML	Submission_2020-11-18_20:57:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zuobin Ma
SpeciesList	scientific name: Oryza sativa; NCBI TaxID: 4530;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-08 21:25:09	ID requested
1	2019-12-08 21:26:49	announced
⏵ 2	2020-11-18 20:57:41	announced	2020-11-19: Update publication information

Ma Z, Wang L, Zhao M, Gu S, Wang C, Zhao J, Tang Z, Gao H, Zhang L, Fu L, Yin Y, He N, Zheng W, Xu Z, iTRAQ proteomics reveals the regulatory response to Magnaporthe oryzae in durable resistant vs. susceptible rice genotypes. PLoS One, 15(1):e0227470(2020) [pubmed]

submitter keyword: Rice, Blast resistance, iTRAQ, Plant proteomics

Zuobin Ma
contact affiliation	Liaoning Academy of Agricultural Sciences
contact email	mazuo1984@163.com
lab head
Zuobin Ma
contact affiliation	Liaoning Academy of Agricultural Sciences
contact email	mazuo1984@163.com
dataset submitter

iProX dataset URI