PXD016638-1

PXD016638 is an original dataset announced via ProteomeXchange.

Title	Dissecting the mechanism of signaling-triggered nuclear export of newly synthesized influenza virus ribonucleoprotein complexes
Description	Influenza viruses (IV) exploit a variety of signaling pathways. Previous studies showed that the Raf/MEK/ERK pathway is functionally linked to nuclear export of viral ribonucleoprotein (vRNP) complexes, suggesting that vRNP export is a signaling induced event. However, the underlying mechanism remained completely enigmatic. Here we have dissected the unknown molecular steps of signaling-driven vRNP export. We identified RSK1 as downstream target of virus-activated ERK signaling. While RSK2 displays an antiviral role, we demonstrate for the first time a virus-supportive function of RSK1, migrating to the nucleus to phosphorylate NP, the major constituent of vRNPs. This drives association with viral M1 at the chromatin, important for vRNP export. Inhibition or knockdown of MEK, ERK or RSK1 caused impaired vRNP export actions of different RSK isoforms.
HostingRepository	PRIDE
AnnounceDate	2020-06-18
AnnouncementXML	Submission_2020-06-17_22:51:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hannes Drexler
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Influenza A virus (A/Wilson-Smith/1933(H1N1)); NCBI TaxID: 381518;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-06 06:30:37	ID requested
⏵ 1	2020-06-17 22:51:33	announced
2	2024-10-22 05:07:25	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: Influenza virus, vRNP complex, vRNP export, kinase signalling, mass spectrometry, proteomics, phosphorylation

Hannes C. A. Drexler
contact affiliation	Max Planck Institute for Molecular Biomedicine Bioanalytical Mass Spectrometry Röntgenstr. 20 48149 Münster Germany
contact email	hannes.drexler@mpi-muenster.mpg.de
lab head
Hannes Drexler
contact affiliation	Bioanalytical Mass Spectrometry
contact email	hannes.drexler@mpi-muenster.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD016638

PRIDE project URI

• PRIDE
  1. PXD016638
     1. Label: PRIDE project
     2. Name: Dissecting the mechanism of signaling-triggered nuclear export of newly synthesized influenza virus ribonucleoprotein complexes