PXD016632-3

PXD016632 is an original dataset announced via ProteomeXchange.

Title	Comprehensive proteome of the adult neural stem cell niche identifies key regulators of adult neurogenesis
Description	Neural stem cells (NSCs) generating new neurons are restricted to few niches in the adult mammalian brain, while the remainder rather promotes gliogenesis. Here we take advantage of the spatial separation of an NSC niche (the subependymal zone) and the region to where the newly generated neurons migrate and integrate (the olfactory bulb). Using state-of-the-art mass spectrometry we present a comprehensive proteomic characterization of these niches compared to a region of the normal brain parenchyma (cerebral cortex) that is not contusive to neurogenesis and new neuron integration. We find unique compositions of regulatory ECM components in the neurogenic niche, with quiescent NSCs as a main source of their local ECM, including the multi-functional enzyme transglutaminase 2 that we identify as crucial for neurogenesis. Atomic force microscopy further corroborates indications from the proteome that neurogenic niches are significantly stiffer than the non-neurogenic parenchyma, highlighting the unique properties of these special niches.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:00:38.077.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jacob Kjell
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-06 05:22:01	ID requested
1	2020-01-27 06:55:45	announced
2	2020-02-16 23:08:37	announced	2020-02-17: Updated publication reference for PubMed record(s): 32032526.
⏵ 3	2024-10-22 05:00:40	announced	2024-10-22: Updated project metadata.

Kjell J, Fischer-Sternjak J, Thompson AJ, Friess C, Sticco MJ, Salinas F, Cox J, Martinelli DC, Ninkovic J, Franze K, Schiller HB, G, ö, tz M, Defining the Adult Neural Stem Cell Niche Proteome Identifies Key Regulators of Adult Neurogenesis. Cell Stem Cell, 26(2):277-293.e8(2020) [pubmed]

10.1016/j.stem.2020.01.002;

submitter keyword: Brain,Stem cells, Extracellular matrix, Neurogenesis

Magdalena Götz
contact affiliation	Pysiological Genomics, LMU Muenchen, Germany
contact email	magdalena.goetz@helmholtz-muenchen.de
lab head
Jacob Kjell
contact affiliation	Karolinska Institutet
contact email	jacob.kjell@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD016632

PRIDE project URI

• PRIDE
  1. PXD016632
     1. Label: PRIDE project
     2. Name: Comprehensive proteome of the adult neural stem cell niche identifies key regulators of adult neurogenesis