PXD016616 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Integrating metabolic and evolutionary engineering for enhanced propionic acid production under cross stress: a multi-omics perspective |
Description | We performed a combination of metabolic engineering (deletion of ldh and poxB and overexpression of mmc) with evolutionary engineering (selection under oxygen stress, acid stress and osmotic stress) in Propionibacterium acidipropionici. The results indicated that the mutants had superior physiological activity, especially the mutant III obtained from P. acidipropionici-Δldh-ΔpoxB+mmc by evolutionary engineering, with 1.5-3.5 times higher growth rates, as well as a 37.1% increase of PA titer and a 37.8% increase PA productivity compared to the wild type. Moreover, the 5.5-fold upregulation of Dps, 2 to 4-fold upregulation of ABC-type glycine betaine transporter and 3-fold upregulation of SOD indicated that these genes were likely in key regulons for the adaptation to abiotic stresses. An approximately 2.5-fold upregulation of mmc was also found. The results showed the multidirectional variation tendency of P. acidipropionici under cross stress and provide in-depth insights into the mechanism of tolerance and high production of PA. |
HostingRepository | PRIDE |
AnnounceDate | 2020-04-27 |
AnnouncementXML | Submission_2020-04-27_00:45:07.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tingting Liu |
SpeciesList | scientific name: Acidipropionibacterium acidipropionici; NCBI TaxID: 1748; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-12-05 06:46:58 | ID requested | |
⏵ 1 | 2020-04-27 00:45:08 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Propionibacterium acidipropionici, Propionic acid, Metabolic and evolutionary engineering, Cross stress, Omics analysis |
Contact List
Tingting Liu |
contact affiliation | College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University |
contact email | 709301295@qq.com |
lab head | |
Tingting Liu |
contact affiliation | Nanjing Tech University |
contact email | 709301295@qq.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD016616 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016616
- Label: PRIDE project
- Name: Integrating metabolic and evolutionary engineering for enhanced propionic acid production under cross stress: a multi-omics perspective