PXD016605-1

PXD016605 is an original dataset announced via ProteomeXchange.

Title	Immediate adaption – Quantitative Proteomics of BCL6 silencing and EGFR TK inhibition
Description	We aimed to identify the response mechanism to EGFR tyrosine kinase inhibition. A431 cells were transfected with mock siRNA or BCL6 siRNA. Cells were then treated with gefitinib. Harvesting was done at 0h, at 24h and at 48h. Two TMT10plex sets were organized as follows: TMT set1: mock 0h (3x, channels 126, 127N and 127C), mock 24h (3x, 128N, 128C and 129N), mock 48h (3x, 129C, 130N, 130C) and internal pooled standard (131) composed from equal aliquots of all samples (mock siRNA and BCL6 siRNA). TMT set2: siBCL6 0h (3x, channels 126, 127N and 127C), siBCL6 24h (3x, 128N, 128C and 129N), siBCL6 48h (3x, 129C, 130N, 130C) and the same internal pooled standard (131) as for set 1.
HostingRepository	PRIDE
AnnounceDate	2020-12-09
AnnouncementXML	Submission_2020-12-09_00:55:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rui Branca
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-04 08:06:22	ID requested
⏵ 1	2020-12-09 00:55:53	announced
2	2024-10-22 05:16:26	announced	2024-10-22: Updated project metadata.

submitter keyword: Human, TMT proteomics, LC-MS, A431 cells, BCL6 siRNA, gefitinib, EGFR inhibition, time course

Janne Lehtiö
contact affiliation	Dept. Oncology Pathology, Karolinska Institutet, Scilifelab, Stockholm, Sweden
contact email	janne.lehtio@ki.se
lab head
Rui Branca
contact affiliation	Clinical Proteomics Unit, Dep. of Oncology-Pathology
contact email	rui.mamede-branca@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD016605

PRIDE project URI

• PRIDE
  1. PXD016605
     1. Label: PRIDE project
     2. Name: Immediate adaption – Quantitative Proteomics of BCL6 silencing and EGFR TK inhibition