PXD016599-1

PXD016599 is an original dataset announced via ProteomeXchange.

Title	CLN6 deficiency causes selective changes in the lysosomal protein composition
Description	Neuronal Ceroid Lipofuscinosis 6 (NCL6) is a neurodegenerative, lysosomal storage disease. It is caused by a deficiency of the transmembrane protein ceroid-lipofuscinosis neuronal protein 6 (CLN6) that resides in the endoplasmic reticulum. In this project the lysosomal proteome changes in NCL6 were investigated. Therefore, lysosomes were purifiedfrom liver tissue of CLN6 knock-out mice, the proteins were labeled by TMT and the CLN6 proteome was compared to wild type controls. Lysosome purification was obtained by either isolation of tritosomes or differential centrifugation generating 20,000 g pellets.
HostingRepository	PRIDE
AnnounceDate	2024-10-28
AnnouncementXML	Submission_2024-10-28_03:44:53.691.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Melanie Thelen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; S-carboxamidoethyl-L-cysteine; phosphorylated residue; monohydroxylated residue; acetylated residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-04 06:39:52	ID requested
⏵ 1	2024-10-28 03:44:54	announced

10.1002/pmic.202100043;

Tuermer A, Mausbach S, Kaade E, Damme M, Sylvester M, Gieselmann V, Thelen M, CLN6 deficiency causes selective changes in the lysosomal protein composition. Proteomics, 21(19):e2100043(2021) [pubmed]

curator keyword: Biomedical

submitter keyword: Cln6, lysosome, nclf

Prof. Volkmar Gieselmann
contact affiliation	Universitaet Bonn Institut fuer Biochemie und Molekularbiologie Nussallee 11 53115 Bonn Germany
contact email	vgieselm@uni-bonn.de
lab head
Melanie Thelen
contact affiliation	Institute for Biochemistry and Molecular Biology, University Bonn, Germany
contact email	mthelen@uni-bonn.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD016599

PRIDE project URI

• PRIDE
  1. PXD016599
     1. Label: PRIDE project
     2. Name: CLN6 deficiency causes selective changes in the lysosomal protein composition