PXD016595 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Kinetic Analysis of DNA Dependent Protein Kinase Catalytic Subunit |
Description | We investigated the dynamics of protein kinase DNA dependent protein kinase catalytic subunit (DNA-PKcs) through hydrogen deuterium exchange mass spectrometry. Over 1100 peptides could be monitored throughout the kinetics experiment, showing the utility of our nanospray HX-MS configuration for ultra-large proteins such as DNA-PKcs. To match the structurally unassigned helices to sequence from the large disordered regions of DNA-PKcs (a.a 2577-2773), we used integrative modelling. Two sequence assignments were possible. The cluster of models with the helix assignment towards the N-terminal side was favored by reduced kinetics of exchange as measured by HX-MS. |
HostingRepository | PRIDE |
AnnounceDate | 2019-12-10 |
AnnouncementXML | Submission_2019-12-09_23:44:58.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Morgan Hepburn |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-12-04 06:33:37 | ID requested | |
⏵ 1 | 2019-12-09 23:45:00 | announced | |
Publication List
Sheff JG, Hepburn M, Yu Y, Lees-Miller SP, Schriemer DC, Nanospray HX-MS configuration for structural interrogation of large protein systems. Analyst, 142(6):904-910(2017) [pubmed] |
Saltzberg DJ, Hepburn M, Pilla KB, Schriemer DC, Lees-Miller SP, Blundell TL, Sali A, SSEThread: Integrative threading of the DNA-PKcs sequence based on data from chemical cross-linking and hydrogen deuterium exchange. Prog Biophys Mol Biol, 147():92-102(2019) [pubmed] |
Keyword List
submitter keyword: HDX-MS, Nanospray HX-MS, DNA-PKcs, Human, |
Contact List
David C. Schriemer |
contact affiliation | Biochemistry & Molecular Biology Cumming School of Medicine University of Calgary, Canada |
contact email | dschriem@ucalgary.ca |
lab head | |
Morgan Hepburn |
contact affiliation | University of Calgary |
contact email | mhoeppne@ucalgary.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD016595 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016595
- Label: PRIDE project
- Name: Kinetic Analysis of DNA Dependent Protein Kinase Catalytic Subunit