PXD016580 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phosphorylation ratio of pT vs pY ERK in MEK phosphorylation reaction determined by mass spectrometry |
Description | The purpose of this experiment is to determine the phosphorylation ratio of pT vs pY ERK present in ERK phosphorylation reaction by activated MEK E203K or MEK WildType. Aliquots were collected at 3 minutes 15 seconds, the time at which the mono-phosphorylated ERK is at its maximal. To determine the ratio between pT and pY ERK, synthetic AQUA peptides corresponding to the pY- and the pT-phosphorylated ERK were spiked into the sample. The two peptides have identical mass but they can be umabiguguously distinguished by their elution times and fragmentation spectra. |
HostingRepository | PRIDE |
AnnounceDate | 2020-01-14 |
AnnouncementXML | Submission_2020-01-13_23:55:19.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Eyan Yeung |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-12-04 03:33:13 | ID requested | |
⏵ 1 | 2020-01-13 23:55:20 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Recombinant, Kinase reaction, LC-MSMS, Orbitrap Fusion Lumos |
Contact List
Martin Wühr |
contact affiliation | Princeton University, Department of Molecular Biology, Lewis-Sigler Institute for Integrative Genomics |
contact email | wuhr@princeton.edu |
lab head | |
Eyan Yeung |
contact affiliation | Princeton University |
contact email | eyanyeung@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016580
- Label: PRIDE project
- Name: Phosphorylation ratio of pT vs pY ERK in MEK phosphorylation reaction determined by mass spectrometry