PXD016561 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Characterization of Fasciola heptica extracellular vesicles recovered by gravity flow |
Description | Parasite-derived extracellular vesicles (EVs) are now at the forefront of helminth research, with evidence indicating that they exhibit immune modulatory activity, presenting a strong case to look at their potential as vaccine candidates. In the present study, Fasciola hepatica derived EVs (FhEVs) were isolated from adult worms using a gravity flow method. These gravity flow derived FhEVs were characterised by proteomics and lectin array. Comparative analysis using the same methods was carried out using FhEVs recovered by differential centrifugation. The immunomodulatory properties of the gravity flow derived FhEVs was determined using bone marrow derived murine dendritic cells (BMDCs), which highlighted that the FhEVs induce a novel phenotype that secreted low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L and SIGNR1) and intracellular markers (SOCS1 and SOCS3). In conclusion, this study demonstrates that FhEVs modulate immune cells, inducing a unique DC phenotype that supresses IL-2 secretion from T-cells. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-11 |
AnnouncementXML | Submission_2024-10-11_03:54:30.007.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD016561 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Krystyna Cwiklinski |
SpeciesList | scientific name: Fasciola hepatica (Liver fluke); NCBI TaxID: 6192; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 5600; LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-12-04 02:12:41 | ID requested | |
⏵ 1 | 2024-10-11 03:54:30 | announced | |
Publication List
10.1371/journal.pntd.0008626; |
10.6019/PXD016561; |
Murphy A, Cwiklinski K, Lalor R, O'Connell B, Robinson MW, Gerlach J, Joshi L, Kilcoyne M, Dalton JP, O'Neill SM, Fasciola hepatica Extracellular Vesicles isolated from excretory-secretory products using a gravity flow method modulate dendritic cell phenotype and activity. PLoS Negl Trop Dis, 14(9):e0008626(2020) [pubmed] |
Keyword List
submitter keyword: Fasciola hepatica |
extracellular vesicles |
excreted-secreted proteins |
gravity flow |
Contact List
John P Dalton |
contact affiliation | Department of Zoology, School of Natural Sciences, National University of Ireland, Galway. |
contact email | johnpius.dalton@nuigalway.ie |
lab head | |
Krystyna Cwiklinski |
contact affiliation | NUI Galway |
contact email | krystyna.cwiklinski@nuigalway.ie |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD016561 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016561
- Label: PRIDE project
- Name: Characterization of Fasciola heptica extracellular vesicles recovered by gravity flow