PXD016558-1

PXD016558 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	EGFR inhibition triggers an adaptive response by co-opting antiviral signaling pathways in lung cancer
Description	HCC827 cells were treated with 0.1 microM erlotinib for 0, 2, 6, and 24 hours. Cell lysates were immunoprecipitated with TBK1 antibody. Antibody enriched protein samples were run on SDS-PAGE gels and submitted to UT Southwestern Proteomics Core Facility for Mass Spectrometry. Protein gel pieces were digested overnight with trypsin (Pierce) following reduction and alkylation with DTT and iodoacetamide (Sigma Aldrich. The samples then underwent solid-phase extraction cleanup with Oasis HLB microelution plates (Waters) and the resulting samples were analyzed by LC-MS-MS, using an Orbitrap Fusion Lumos mass spectrometer (Thermo Electron) coupled to an Ultimate 3000 RSLC-Nano liquid chromatography systems (Dionex).
HostingRepository	MassIVE
AnnounceDate	2019-12-03
AnnouncementXML	Submission_2019-12-03_12:49:59.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mohammad
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-03 12:45:30	ID requested
⏵ 1	2019-12-03 12:50:00	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: EGFR inhibition, therapeutic resistance, Type I interferons, TBK1, IRF3,RIG-I, lung cancer, NSCLC, erlotinib, EGFR wild type, Anifrolumab

submitter keyword: EGFR inhibition, therapeutic resistance, Type I interferons, TBK1, IRF3,RIG-I, lung cancer, NSCLC, erlotinib, EGFR wild type, Anifrolumab

Contact List

Amyn A. Habib
contact affiliation	University of Texas Southwestern Medical Center, North Texas VA Healthcare System, Mail Code 151, 4500 South Lancaster Road, Dallas TX 75216
contact email	Amyn.Habib@UTSouthwestern.edu
lab head
Mohammad
contact affiliation	UT Southwestern Medical center
contact email	mohammad.goodarzi@utsouthwestern.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000084654/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000084654/