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PXD016333-1

PXD016333 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComparative proteomic analysis of Leishmania parasite isolates obtained from visceral and cutaneous leishmaniases patients
DescriptionAs a vector-borne disease, leishmaniasis is caused by a parasitic protozoans of leishmania genus and transmitted by female Phlebotomine sandflies. Depending on the body location where immotile form of the parasite namely amastigote is proliferated, three main clinical forms as cutaneous, muco-cutaneous and visceral leishmaniases are defined. While manifestation of cutaneous leishmaniasis is skin lesions on the exposed part of the body, enlarged lymph nodes, spleen or liver along with fever, fatigue and weight loss are the symptoms of visceral leishmaniasis. The most dangerous form is visceral leishmaniasis since it may end up with fatalities if patients are not treated. The purpose of this study was to investigate the difference between the protein expression profiles of leishmania isolates obtained from visceral and cutaneous leishmaniasis patients. To compare two sample groups to each other genetically, L.infantum was chosen since it causes both visceral and cutaneous leishmaniasis. Additionally, another sample group as cutaneous leishmaniasis caused by L.tropica was included to make the comparison both intra- and interspecies level. For protein profiling, both gel-based and gel-free proteomic approaches were carried out. In brief, a total of 15 samples, 5 from each group, were separated on pI 3-10 2D-PAGE gel. Additionally, 9 of those 15 samples, 3 from each group, were analyzed according to qualitative shotgun proteomics method and differential proteins were determined by drawing venn diagram.
HostingRepositoryPRIDE
AnnounceDate2024-10-11
AnnouncementXMLSubmission_2024-10-11_03:54:19.558.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD016333
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMelike Dinc
SpeciesList scientific name: Leishmania infantum; NCBI TaxID: 5671; scientific name: Leishmania tropica; NCBI TaxID: 5666;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ XL
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-11-19 01:54:01ID requested
12024-10-11 03:54:20announced
Publication List
10.6019/PXD016333;
10.1017/s0031182021001967;
Din, ç M, Yal, ç, ı, n T, Ç, avu, ş İ, Ö, zbilgin A, parasites isolated from visceral and cutaneous leishmaniasis patients. Parasitology, 149(3):298-305(2022) [pubmed]
Keyword List
submitter keyword: leishmania, parasite, leishmaniasis, neglected tropical disease, sandfly,Shotgun proteomics, leishmania infantum, leishmania tropica
Contact List
Prof. Dr. Talat YALCIN
contact affiliationFaculty of Science, Department of Chemistry, B-205 İzmir Institute of Technology (IzTech)
contact emailtalatyalcin@iyte.edu.tr
lab head
Melike Dinc
contact affiliationIzmir Institute of Technology
contact emailmelikedinc@iyte.edu.tr
dataset submitter
Full Dataset Link List
Dataset FTP location
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