PXD016333-1

PXD016333 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic analysis of Leishmania parasite isolates obtained from visceral and cutaneous leishmaniases patients
Description	As a vector-borne disease, leishmaniasis is caused by a parasitic protozoans of leishmania genus and transmitted by female Phlebotomine sandflies. Depending on the body location where immotile form of the parasite namely amastigote is proliferated, three main clinical forms as cutaneous, muco-cutaneous and visceral leishmaniases are defined. While manifestation of cutaneous leishmaniasis is skin lesions on the exposed part of the body, enlarged lymph nodes, spleen or liver along with fever, fatigue and weight loss are the symptoms of visceral leishmaniasis. The most dangerous form is visceral leishmaniasis since it may end up with fatalities if patients are not treated. The purpose of this study was to investigate the difference between the protein expression profiles of leishmania isolates obtained from visceral and cutaneous leishmaniasis patients. To compare two sample groups to each other genetically, L.infantum was chosen since it causes both visceral and cutaneous leishmaniasis. Additionally, another sample group as cutaneous leishmaniasis caused by L.tropica was included to make the comparison both intra- and interspecies level. For protein profiling, both gel-based and gel-free proteomic approaches were carried out. In brief, a total of 15 samples, 5 from each group, were separated on pI 3-10 2D-PAGE gel. Additionally, 9 of those 15 samples, 3 from each group, were analyzed according to qualitative shotgun proteomics method and differential proteins were determined by drawing venn diagram.
HostingRepository	PRIDE
AnnounceDate	2024-10-11
AnnouncementXML	Submission_2024-10-11_03:54:19.558.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD016333
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Melike Dinc
SpeciesList	scientific name: Leishmania infantum; NCBI TaxID: 5671; scientific name: Leishmania tropica; NCBI TaxID: 5666;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ XL

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-19 01:54:01	ID requested
⏵ 1	2024-10-11 03:54:20	announced

10.6019/PXD016333;

10.1017/s0031182021001967;

Din, ç M, Yal, ç, ı, n T, Ç, avu, ş İ, Ö, zbilgin A, parasites isolated from visceral and cutaneous leishmaniasis patients. Parasitology, 149(3):298-305(2022) [pubmed]

submitter keyword: leishmania, parasite, leishmaniasis, neglected tropical disease, sandfly,Shotgun proteomics, leishmania infantum, leishmania tropica

Prof. Dr. Talat YALCIN
contact affiliation	Faculty of Science, Department of Chemistry, B-205 İzmir Institute of Technology (IzTech)
contact email	talatyalcin@iyte.edu.tr
lab head
Melike Dinc
contact affiliation	Izmir Institute of Technology
contact email	melikedinc@iyte.edu.tr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016333
     1. Label: PRIDE project
     2. Name: Comparative proteomic analysis of Leishmania parasite isolates obtained from visceral and cutaneous leishmaniases patients