PXD016322 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Human bone marrow comprise atypical adipocytes with specific lipid metabolism |
Description | Under caloric restriction, bone marrow adipocytes (BM-Ad) do not decrease in size conversely to white adipocytes, suggesting their unique metabolic properties. We compared human primary BM-Ad with paired subcutaneous adipocytes (SC-Ad) using proteomic and lipidomic approaches. We found that while SC-Ad and BM-Ad share similar morphological features, they possess distinct lipid metabolism. BM-Ad shows enrichment in proteins involved in cholesterol metabolism correlating with increased free cholesterol content while proteins involved in lipolysis were downregulated. In particular, monoacylglycerol lipase expression was strongly reduced in BM-Ad, leading to accumulation of monoacylglycerol. Consequently, basal and induced lipolytic responses were absent in BM-Ad, affirming their differences in metabolic fitness upon caloric restriction. These specific metabolic features are not recapitulated in vitro using common protocols to differentiate bone marrow mesenchymal stem cells. Thus, contrary to classical SC-Ad, BM-Ad display a specific lipid metabolism, as they are devoid of lipolytic activity and exhibit a cholesterol-orientated metabolism. |
HostingRepository | PRIDE |
AnnounceDate | 2020-01-29 |
AnnouncementXML | Submission_2020-01-29_03:53:04.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Manuelle Ducoux-Petit |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-11-18 07:32:58 | ID requested | |
⏵ 1 | 2020-01-29 03:53:05 | announced | |
2 | 2020-02-17 03:23:10 | announced | 2020-02-17: Updated publication reference for PubMed record(s): 31995765. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Bone Marrow adipocytes, human, label free quantificatio, LC-MSMS |
Contact List
Catherine Muller |
contact affiliation | Team: Microenvironnement, Cancer and Adipocytes Institute of Pharmacology and Structural Biology BP64182 205 route de Narbonne 31077 Toulouse Cedex04 France |
contact email | muller@ipbs.fr |
lab head | |
Manuelle Ducoux-Petit |
contact affiliation | CNRS |
contact email | manuelle.ducoux@ipbs.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016322
- Label: PRIDE project
- Name: Human bone marrow comprise atypical adipocytes with specific lipid metabolism