PXD016303-1

PXD016303 is an original dataset announced via ProteomeXchange.

Title	Alterations of brain quantitative proteomics profiling revealed the molecular mechanisms of diosgenin against cerebral ischemia reperfusion effects
Description	Diosgenin (DIO), the starting material for the synthesis of steroidal anti-inflammatory drugs in pharmaceutical industry, has been previously demonstrated to display pharmaceutical effects against cerebral ischemic reperfusion (I/R). However, the alterations of brain proteome profiles underlying this treatment remain elusive. In the present study, the proteomics analysis of the brain tissues from I/R rats after DIO treatment was performed by an integrated TMT-based quantitative proteomic approach coupled with LC-MS/MS technology. A total of 5043 proteins were identified, of which 58 common differentially expressed proteins (DEPs) were significantly dysregulated in comparison between Sham verse I/R and I/R verse DIO. The 8 validated proteins including EPG5, STAT2, CPT1A, EIF2AK2, GGCT, HIKESHI, TNFAIP8, and EMC6 by qPCR and Western blotting consistently supported the TMT-based proteomic results, which were mainly associated with autophagy and inflammation response. Considering the anti-inflammatory characters of DIO, the biological functions of STAT2 and HIKESHI that are the probably direct anti-inflammatory targets were further investigated during the course of I/R treated with DIO. In addition, the combination of verified STAT2 and HIKESHI in peripheral blood samples from stroke patients resulted in AUC value of 0.765 with P<0.004 to distinguish stroke patients from healthy controls. Taken together, the current findings firstly mapped comprehensive proteomic changes after I/R treated with DIO to better decipher the molecular mechanisms mainly based on anti-inflammatory aspect underlying this therapeutic effect, providing a foundation for developing potentially therapeutic targets of anti-I/R of DIO and clinically prognostic biomarkers of stroke
HostingRepository	iProX
AnnounceDate	2019-11-18
AnnouncementXML	Submission_2020-11-17_23:50:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xinxin Zhang
SpeciesList	scientific name: Rattus rattus; NCBI TaxID: 10117;
ModificationList	TMT6plex reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-17 20:23:43	ID requested
⏵ 1	2020-11-17 23:50:26	announced

Zhang X, Wang X, Khurm M, Zhan G, Zhang H, Ito Y, Guo Z, Alterations of Brain Quantitative Proteomics Profiling Revealed the Molecular Mechanisms of Diosgenin against Cerebral Ischemia Reperfusion Effects. J Proteome Res, 19(3):1154-1168(2020) [pubmed]

submitter keyword: Diosgenin, cerebral ischemic reperfusion, anti-inflammatory, STAT2, HIKESHI

Zengjun Guo
contact affiliation	College of Pharmacy, Xi’an Jiaotong University
contact email	guozj@mail.xjtu.edu.cn
lab head
Xinxin Zhang
contact affiliation	College of Pharmacy, Xi'an Jiaotong University
contact email	zhangxinxin360@126.com
dataset submitter

iProX dataset URI