PXD016213-2

PXD016213 is an original dataset announced via ProteomeXchange.

Title	No evidence for major spacial differences within the microbial communities in anaerobic digestion plants under different feeding and stirring regimes
Description	In anaerobic digestion plants (ADP) homogenization of the feed, the fermenter content and the microbial communities represents a precondition for effective and robust biogas production,but also a major energy consumer. For a 850 m3 agricultural AD-P equipped with eight sampling ports we investigated whether different feeding and stirring regimes enable a sufficient homogenization of the microbial communities using metaproteome and TRFLP analysis. Systematic comparison of the samples by scatter plots and students t-test revealed only a limited number of slightly changed metaproteins, taxonomies and biological processes, indicating no systematic differences between the microbial communities in center and rim as well as between top and bottom. However, comparison of the amount of shared identified metaproteins between the sample ports showed minor variation, which might be correlated with the applied stirring strategy. In sum, the applied stirring and feeding conditionswere sufficient to homogenize the microbial communities in AD-Ps largely.
HostingRepository	PRIDE
AnnounceDate	2021-04-28
AnnouncementXML	Submission_2021-04-28_01:21:08.429.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Robert Heyer
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-11 00:52:24	ID requested
1	2021-04-28 01:20:22	announced
⏵ 2	2021-04-28 01:21:09	announced	2021-04-28: Updated publication reference for PubMed record(s): 32629381.
3	2024-10-22 05:21:29	announced	2024-10-22: Updated project metadata.

Heyer R, Klang J, Hellwig P, Schallert K, Kress P, Huelsemann B, Theuerl S, Reichl U, Benndorf D, Impact of feeding and stirring regimes on the internal stratification of microbial communities in the fermenter of anaerobic digestion plants. Bioresour Technol, 314():123679(2020) [pubmed]

curator keyword: Biological

submitter keyword: anaerobic digestion

biogas, stirring, microbial communities, TRFLP, metaproteomics

Dirk Benndorf
contact affiliation	Otto-von-Guericke Universität Magdeburg
contact email	benndorf@mpi-magdeburg.mpg.de
lab head
Robert Heyer
contact affiliation	OvGU
contact email	heyer@mpi-magdeburg.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016213
     1. Label: PRIDE project
     2. Name: No evidence for major spacial differences within the microbial communities in anaerobic digestion plants under different feeding and stirring regimes