PXD016212-1
PXD016212 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Long-lived Metabolic Enzymes in the Crystallin Lens Identified by Pulse-labeling of Mice and Mass Spectrometry |
| Description | The lenticular fiber cells are comprised of extremely long-lived proteins while still maintaining an active biochemical state. Dysregulation of these activities has been implicated in age-related cataracts, and other lens diseases. However, the lenticular protein dynamics underlying health and disease is unclear. We sought to measure the global protein turnover rates in the eye using dietary nitrogen-15 (15N)-labeling of mice between 3 and 15 weeks of age. By performing mass spectrometry we measured the 14N- to 15N-peptide ratios of hundreds of lens proteins, including Crystallin, Aquaporin, Collagen and Laminin of the lens capsule, and enzymes that catalyze glycolysis as well as oxidation and reduction reactions. Direct comparison of lens cortex versus nucleus longevity revealed little or no 15N-protein contents in most proteins in the lens nucleus, while there were a broad range of 14N/15N ratios of proteins in the cortex, including crystallin isoforms. Unexpectedly, like the crystallin proteins, many enzymes were also exceedingly long-lived, including those present at relatively high abundance in the nucleus. The slow replacement of these enzymes in spite of young age of the mice suggests their potential roles in age-related metabolic changes in the lens. |
| HostingRepository | MassIVE |
| AnnounceDate | 2019-12-13 |
| AnnouncementXML | Submission_2019-12-13_05:20:47.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Jeffrey N. Savas |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | Carbamidomethyl |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2019-11-08 13:20:21 | ID requested | |
| ⏵ 1 | 2019-12-13 05:20:48 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Lens Proteomics, 15N labeling, Protein Turnover |
Contact List
| Jing Jin | |
|---|---|
| contact affiliation | Northwestern University |
| contact email | jing.jin@northwestern.edu |
| lab head | |
| Jeffrey N. Savas, PhD | |
| contact affiliation | Department of Neurology Northwestern University |
| contact email | jeffrey.savas@northwestern.edu |
| lab head | |
| Jeffrey N. Savas | |
| contact affiliation | Northwestern University |
| contact email | jeffrey.savas@northwestern.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000084566/ |
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