PXD016136 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The endoplasmic reticulum-associated mRNA-binding protein ERBP1 from Trypanosoma brucei |
Description | Kinetoplastids rely heavily on post-transcriptional mechanisms for control of gene expression, and on RNA-binding proteins that regulate mRNA splicing, translation and decay. Trypanosoma brucei ERBP1 (Tb927.10.14150) and ERBP2 (Tb927.9.9550) were previously identified as mRNA binding proteins that lack canonical RNA-binding domains. We here show that ERBP1 is associated with the endoplasmic reticulum, like ERBP2, and that the two proteins interact in vivo. Loss of ERBP1 from bloodstream-form T. brucei initially resulted in a growth defect but proliferation was restored after more prolonged cultivation. Results from a pull-down of tagged ERBP1 suggest that it preferentially binds to ribosomal protein mRNAs. The ERBP1 sequence resembles that of Saccharomyces cerevisiae Bfr1, which also localises to the endoplasmic reticulum and binds to ribosomal protein mRNAs. However, unlike Bfr1, ERBP1 does not bind to mRNAs encoding secreted proteins, and it is also not recruited to stress granules after starvation. |
HostingRepository | PRIDE |
AnnounceDate | 2020-05-26 |
AnnouncementXML | Submission_2020-05-26_15:15:33.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Thomas Ruppert |
SpeciesList | scientific name: Trypanosoma brucei; NCBI TaxID: 5691; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Velos; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-11-04 05:17:28 | ID requested | |
⏵ 1 | 2020-05-26 15:15:34 | announced | |
Publication List
Bajak K, Leiss K, Clayton CE, Erben E, . PeerJ, 8():e8388(2020) [pubmed] |
Keyword List
submitter keyword: RNA-binding proteins |
Trypanosoma brucei |
post-transcripcional control |
Contact List
Thomas Ruppert |
contact affiliation | mass spectrometry and proteomics, Centre for molecular biology at the university Heidelberg (ZMBH), Im Neuenheimer Feld 282, D-69120 Heidelberg, Germany |
contact email | t.ruppert@zmbh.uni-heidelberg.de |
lab head | |
Thomas Ruppert |
contact affiliation | ZMBH, Im Neuenheimer Feld 282, 69122 Heidelberg |
contact email | t.ruppert@zmbh.uni-heidelberg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016136
- Label: PRIDE project
- Name: The endoplasmic reticulum-associated mRNA-binding protein ERBP1 from Trypanosoma brucei