PXD016135 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomics of regenerated tissue in response to a titanium implant with a bioactive surface in a rat tibial defect model |
Description | Background: Due to their excellent mechanical and biocompatibility properties, titanium-based implants are successfully used as biomedical devices. However, when new bone formation fails for different reasons, impaired fracture healing will become a clinical problem and will affect the patient's quality of life. This study proposes to design a new bioactive surface of titanium implants with a synergetic PEG biopolymer-based composition for gradual delivery of growth factors (FGF2, VEGF, and BMP4) during bone healing. Methods: The optimal architecture of non-cytotoxic polymeric coatings deposited by dip coating under controlled parameters was assessed both in cultured cells and on rat in vivo animal model (100% viability). Results: Notably, the titanium adsorbed polymer matrix induced an improved healing process when compared with the individual action of each biomolecules. High-performance mass spectrometry analysis demonstrated that recovery after a traumatic event is governed by specific differentially regulated proteins, acting in a coordinated response to the external stimulus. Predicted protein interactions shown by STRING analysis were well organized in hub-based networks related with response to chemical, wound healing and response to stress pathways. Conclusions: The described functional polymer coatings of the titanium implants demonstrated the significant improvement of bone healing process after injury. |
HostingRepository | PRIDE |
AnnounceDate | 2021-04-29 |
AnnouncementXML | Submission_2021-04-29_10:31:44.108.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD016135 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Felicia Antohe |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | residues isobaric at 113.084064 Da; Oxidation; L-tyrosine residue; Carbamidomethyl |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-11-04 05:17:27 | ID requested | |
⏵ 1 | 2021-04-29 10:31:45 | announced | |
Publication List
Boteanu RM, Suica VI, Ivan L, Safciuc F, Uyy E, Dragan E, Croitoru SM, Grumezescu V, Chiritoiu M, Sima LE, Vlagioiu C, Socol G, Antohe F, Proteomics of regenerated tissue in response to a titanium implant with a bioactive surface in a rat tibial defect model. Sci Rep, 10(1):18493(2020) [pubmed] |
Keyword List
submitter keyword: bone healing, growth factors, titanium implant, MS-based proteomics |
Contact List
Felicia Antohe |
contact affiliation | Proteomics Department, Institute of Cellular Biology and Pathology "N. Simionescu", Romania |
contact email | felicia.antohe@icbp.ro |
lab head | |
Felicia Antohe |
contact affiliation | Institute of Cellular Biology and Pathology "N. Simionescu" |
contact email | felicia.antohe@icbp.ro |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD016135 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016135
- Label: PRIDE project
- Name: Proteomics of regenerated tissue in response to a titanium implant with a bioactive surface in a rat tibial defect model