PXD016105-1

PXD016105 is an original dataset announced via ProteomeXchange.

Title	Proteome of naive and TCR activated wild-type, Myc-deficient and Slc7a5-deficient T cells
Description	T cell clonal expansion and differentiation are critically dependent on the transcription factor c-Myc (Myc). Herein we use quantitative mass-spectrometry to reveal how Myc controls antigen receptor driven cell growth and proteome restructuring in CD4+ and CD8+ T cells. Quantitative proteomics was performed on naive wild-type (WT) and 24 hr T cell receptor (TCR) activated Myc WT and Myc-deficient T cells. Analysis of copy numbers per cell of >7000 proteins provides new understanding of the selective role of Myc in controlling the protein machinery that shapes T cell fate. The data identify both Myc dependent and independent metabolic processes in immune activated T cells. We uncover that a primary function of Myc is to induce amino acid transporter expression. Quantitative proteomics of 24 hr TCR activated Slc7a5 WT and Slc7a5-deficient CD4 T cells reveals that loss of a single Myc-controlled amino transporter, Slc7a5, can effectively phenocopy the impact of Myc deletion. This study thus provides a comprehensive map of how Myc selectively shapes T cell phenotypes and reveals that Myc induction of amino acid transport is pivotal for subsequent bioenergetic and biosynthetic programs.
HostingRepository	PRIDE
AnnounceDate	2019-11-25
AnnouncementXML	Submission_2019-11-25_05:07:32.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julia Marchingo
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-01 03:51:38	ID requested
⏵ 1	2019-11-25 05:07:33	announced
2	2020-05-18 07:05:32	announced	2020-05-18: Updated publication reference for PubMed record(s): 32022686.
3	2024-10-22 04:58:23	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Mouse, T lymphocyte, LC-MSMS, Myc, Slc7a5, amino acid transport

Doreen Ann Cantrell
contact affiliation	Cell Signalling and Immunology Division, School of Life Sciences, University of Dundee
contact email	d.a.cantrell@dundee.ac.uk
lab head
Julia Marchingo
contact affiliation	School of Life Sciences, University of Dundee
contact email	j.m.marchingo@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD016105

PRIDE project URI

• PRIDE
  1. PXD016105
     1. Label: PRIDE project
     2. Name: Proteome of naive and TCR activated wild-type, Myc-deficient and Slc7a5-deficient T cells