PXD016099-2

PXD016099 is an original dataset announced via ProteomeXchange.

Title	Impact of gut microbiota on the m6A epitranscriptome in mouse cecum and liver tissues
Description	The intestinal microbiota modulates host physiology and gene expression via mechanisms that are not fully understood. A recently discovered layer of gene expression regulation is N6-methyladenosine (m6A) and N6,2′ -O-dimethyladenosine (m6Am) modifications of mRNA. To unveil if these epitranscriptomic marks are affected by the gut microbiota, we performed methylated RNA-immunoprecipitation and sequencing (MeRIP-seq) to examine m6A-modifications in transcripts of mice displaying either a conventional, or a modified, or no gut microbiota and discovered that the microbiota has a strong influence on m6A- modifications in the cecum, and also, albeit to a lesser extent, in the liver, affecting pathways related to metabolism, inflammatory and antimicrobial responses . We furthermore analysed expression levels of several known writer and eraser enzymes and found the methyltransferase Mettl16 to be downregulated in absence of a microbiota. As a consequence, one of its targets, the S-adenosyl methionine synthase Mat2a was less expressed in mice without gut flora. We furthermore show that distinct commensal bacteria, Akkermansia muciniphila, Lactobacillus plantarum can affect specific m6A modifications. Together, we report here epitranscriptomic modifications as an additional level of interaction in the complex interplay between commensal bacteria and their host.
HostingRepository	PRIDE
AnnounceDate	2020-02-17
AnnouncementXML	Submission_2020-05-18_06:04:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MARIETTE MATONDO
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	ubiquitinylated lysine; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-31 09:59:45	ID requested
1	2020-02-17 10:01:47	announced
⏵ 2	2020-05-18 06:04:10	announced	2020-05-18: Updated publication reference for PubMed record(s): 32165618.
3	2024-10-22 04:58:24	announced	2024-10-22: Updated project metadata.

Jabs S, Biton A, B, é, cavin C, Nahori MA, Ghozlane A, Pagliuso A, Span, ò G, Gu, é, rineau V, Touboul D, Giai Gianetto Q, Chaze T, Matondo M, Dillies MA, Cossart P, A epitranscriptome of mouse cecum and liver. Nat Commun, 11(1):1344(2020) [pubmed]

submitter keyword: microbiota, mouse

Mariette Matondo
contact affiliation	Institut Pasteur
contact email	mariette.matondo@pasteur.fr
lab head
MARIETTE MATONDO
contact affiliation	Pasteur
contact email	mariette.matondo@pasteur.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016099
     1. Label: PRIDE project
     2. Name: Impact of gut microbiota on the m6A epitranscriptome in mouse cecum and liver tissues