PXD016092 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Spodoptera frugiperda kinetochore co-immunoprecipitations |
Description | Accurate chromosome segregation requires assembly of the multiprotein kinetochore complex at centromeres. In most eukaryotes, kinetochore assembly is primed by the histone H3 variant CenH3, which physically interacts with components of the inner kinetochore constitutive-centromere-associated-network (CCAN). Unexpected to its critical function, previous work identified that select eukaryotic lineages, including several insects, have lost CenH3, while having retained homologs of the CCAN. These findings imply alternative CCAN assembly pathways in these organisms that function in CenH3-independent manners. Here, we study the composition and assembly of CenH3-independent kinetochores of Lepidoptera. We show that lepidopteran kinetochores consist of previously identified CCAN homologs as well as additional components including a divergent CENP-T homolog, which are required for accurate mitotic progression. Our study focuses on CENP-T that we find both necessary and sufficient to recruit the Mis12 outer kinetochore complex. In addition, CRISPR-mediated gene editing in Bombyx mori establishes an essential function of CENP-T in vivo. Finally, the retention of CENP-T homologs in other independently-derived CenH3-deficient insects indicates a conserved mechanism of kinetochore assembly between these lineages. Our study provides the first functional insights into CCAN-based kinetochore assembly pathways that function independently of CenH3, thus contributing to the emerging picture of an unexpected plasticity to build a kinetochore. |
HostingRepository | PRIDE |
AnnounceDate | 2020-02-18 |
AnnouncementXML | Submission_2020-02-17_21:07:06.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Valentin SABATET |
SpeciesList | scientific name: Bombyx mori (Silk moth); NCBI TaxID: 7091; scientific name: Spodoptera frugiperda; NCBI TaxID: 7108; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF-X; Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-10-31 02:18:33 | ID requested | |
⏵ 1 | 2020-02-17 21:07:08 | announced | |
Publication List
Cortes-Silva N, Ulmer J, Kiuchi T, Hsieh E, Cornilleau G, Ladid I, Dingli F, Loew D, Katsuma S, Drinnenberg IA, CenH3-Independent Kinetochore Assembly in Lepidoptera Requires CCAN, Including CENP-T. Curr Biol, 30(4):561-572.e10(2020) [pubmed] |
Keyword List
submitter keyword: Spodoptera, Frugiperda, Bombix, kinetochore |
Contact List
Damarys Loew |
contact affiliation | Head of the Curie Institute Mass Spectrometry Platform |
contact email | damarys.loew@curie.fr |
lab head | |
Valentin SABATET |
contact affiliation | Curie Institute |
contact email | valentin.sabatet@curie.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016092
- Label: PRIDE project
- Name: Spodoptera frugiperda kinetochore co-immunoprecipitations