PXD016053 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The M-phase regulatory phosphatase PP2A-B55δ opposes protein kinase A on Arpp19 to initiate meiotic division |
Description | Oocytes are held in meiotic prophase for prolonged periods until hormonal signals trigger meiotic divisions. Key players of M-phase entry are the opposing Cdk1 kinase and PP2AB55δ phosphatase. In Xenopus, the protein Arpp19, phosphorylated at serine 67 by Greatwall, plays an essential role in inhibiting PP2A-B55δ, promoting Cdk1 activation. Furthermore Arpp19 has an earlier role in maintaining the prophase arrest through a second serine (S109) phosphorylated by PKA. Prophase release, induced by progesterone, relies on Arpp19 dephosphorylation at S109, owing to an unknown phosphatase. Here we identified this phosphatase as PP2A-B55δ. In prophase, PKA and PP2A-B55δ are simultaneously active, suggesting the presence of other important targets for both enzymes. The drop in PKA activity induced by progesterone decreases S109 phosphorylation, unlocking the prophase block. Hence, PP2A-B55δ acts critically on Arpp19 on two distinct sites, opposing PKA and Greatwall to orchestrate the prophase release and M-phase entry. |
HostingRepository | PRIDE |
AnnounceDate | 2019-10-28 |
AnnouncementXML | Submission_2019-10-28_09:13:35.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Laurent Lignières |
SpeciesList | scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-10-28 08:12:11 | ID requested | |
⏵ 1 | 2019-10-28 09:13:36 | announced | |
2 | 2024-10-22 04:58:10 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Meiotic division, Arpp19, PP2A, PKA, Greatwall, Cdk1, Xenopus oocyte |
Contact List
Sigrid Baumgarten |
contact affiliation | Proteomic facility of Institut Jacques Monod, UMR7592 (CNRS and Paris University), 75013 Paris, France |
contact email | sigrid.baumgarten@ijm.fr |
lab head | |
Laurent Lignières |
contact affiliation | Institut Jacques Monod |
contact email | laurent.lignieres@ijm.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD016053 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016053
- Label: PRIDE project
- Name: The M-phase regulatory phosphatase PP2A-B55δ opposes protein kinase A on Arpp19 to initiate meiotic division