PXD016034 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A rare-codon-based translational program of cell proliferation |
Description | The codon usage of mRNAs controls the speed of translation elongation, which is primarily determined by the abundance of cognate tRNAs. By profiling mRNA expression around the cell cycle we found that mRNAs that are relatively upregulate in the G2/M phase are enriched in rare codons. To understand the impact of this codon bias on translation, we have cultured NIH 3T3 cells with different concentrations of fetal calf serum (FCS), 1, 2, 5, and 10%, respectively, to induce distinct proliferation rates and thus distinct proportions of cells in the culture in the G2/M phase. We then estimated the levels of all proteins and mRNAs, and the change in translation efficiency (proteins per mRNA) in highly (10% FCS) relatively to less highly proliferating cells (lower FCS concentrations). |
HostingRepository | PRIDE |
AnnounceDate | 2020-01-21 |
AnnouncementXML | Submission_2020-01-21_02:14:12.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Katarzyna Buczak |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-10-28 02:11:22 | ID requested | |
⏵ 1 | 2020-01-21 02:14:13 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: Cell proliferation, translation control, codon usage, rare codons, translation elongation, tRNA pools, LFQ |
Contact List
Alexander Schmidt |
contact affiliation | Biozentrum, University of Basel |
contact email | alex.schmidt@unibas.ch |
lab head | |
Katarzyna Buczak |
contact affiliation | Biozentrum, University of Basel |
contact email | katarzyna.buczak@unibas.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016034
- Label: PRIDE project
- Name: A rare-codon-based translational program of cell proliferation