PXD016017 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A Pluripotent Stem Cell-Derived Matrix with Potent Osteoregenerative Capabilities |
Description | Approximately 10% of fractures that occur in the United States annually will not heal without intervention. This figure is rising due to an aging population and a rise in conditions that impair bone healing. Current treatments can be marginally effective, costly, and some have adverse effects. A safe and manufacturable material that mimics anabolic bone is the holy grail of bone tissue engineering, but achieving this is challenging. Adult stem cells, especially mesenchymal stem cells (MSCs), are excellent candidates for engineering bone, but the lack of reproducibility due to donor variation and culture protocols have hindered progress in this area. Herein, we describe an MSC line generated from induced pluripotent stem cells with remarkable osteogenic properties. The cells can be induced to generate large amounts of osteogenic extracellular matrix (ihMatrix) under monolayer culture conditions. When pure ihMatrix is implanted into calvarial defects in mice, it has intrinsic osteogenic activity that significantly surpasses the efficacy of bone morphogenic protein 2 resulting in complete healing of defects in 4 weeks through a mechanism of action mediated in part by collagen VI and XII. Based on these findings, we propose that ihMatrix could represent a superior replacement for the current gold standards, autograft and BMP products, used commonly in bone tissue engineering. |
HostingRepository | PRIDE |
AnnounceDate | 2020-07-02 |
AnnouncementXML | Submission_2020-07-01_22:28:07.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD016017 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Carl Gregory |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Oxidation; Carbamidomethyl |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-10-25 02:34:38 | ID requested | |
⏵ 1 | 2020-07-01 22:28:08 | announced | |
Publication List
McNeill EP, Zeitouni S, Pan S, Haskell A, Cesarek M, Tahan D, Clough BH, Krause U, Dobson LK, Garcia M, Kung C, Zhao Q, Saunders WB, Liu F, Kaunas R, Gregory CA, Characterization of a pluripotent stem cell-derived matrix with powerful osteoregenerative capabilities. Nat Commun, 11(1):3025(2020) [pubmed] |
Keyword List
submitter keyword: Stem Cell, Matrix |
Contact List
Carl A. Gregory |
contact affiliation | Department of Molecular and Cellular Medicine, Institute for Regenerative Medicine, Texas A&M Health Science Center, College Station, TX, 77843, USA. |
contact email | cgregory@tamu.edu |
lab head | |
Carl Gregory |
contact affiliation | Texas A&M Health Science Center |
contact email | cgregory@tamu.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD016017 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016017
- Label: PRIDE project
- Name: A Pluripotent Stem Cell-Derived Matrix with Potent Osteoregenerative Capabilities